MENA 3100 261 10 Optical microscopy We want

The simple microscope Antony van Leeuwenhoek (1632− 1723)

The compound microscope ca. 1595 Hans Janssen Robert Hooke (1635− 1703) Micrographia, 1664

Don’t get too hang up in the ray tracing

Compound microscope for transmitted light

Inverted compound microscope for reflected light

www. microscopy-analysis. com/files/ jwiley_microscopy/2006_Sept_Hammond. pdf

Illuminating system Specimen Condenser lens Aperture diaphragm Field diaphragm Filament

Illuminating system Specimen Condenser lens Aperture diaphragm Köhler illumination Uniform specimen illumination Field diaphragm

Imaging system Eyepiece Okular Tube lens Objective Specimen

$Refraction Kromatisk abberation$

Numerical aperture (NA) NA = nsina Resolution R = 0, 61 l/NA Rayleigh criterion

$The limiting factor: Diffraction NA = nsina Oil immersion$

More is needed than just the objective Condenser system Eyepice Ocular (okular)

$Amorphous and cubic: Optically isotropic, one refracive index Hexagonal, trigonal, tetragonal: Optically anisotropic, two$

Optically active crystals in the microscope

Remember that geologists often call their book on microscopy on thin sections for optical

Slides: 28

Download presentation

MENA 3100, 26/1 -10 Optical microscopy We want to see more details Magnification Resolution Contrast Simple microscope

The simple microscope Antony van Leeuwenhoek (1632− 1723)

The compound microscope ca. 1595 Hans Janssen Robert Hooke (1635− 1703) Micrographia, 1664

Don’t get too hang up in the ray tracing

Stereo microscope

Compound microscope for transmitted light

Inverted compound microscope for reflected light

www. microscopy-analysis. com/files/ jwiley_microscopy/2006_Sept_Hammond. pdf

Illuminating system Specimen Condenser lens Aperture diaphragm Field diaphragm Filament

Illuminating system Specimen Condenser lens Aperture diaphragm Köhler illumination Uniform specimen illumination Field diaphragm Controll illuminated area Field diaphragm Filament Aperture diaphragm Controll angle of the cone of light

Imaging system Eyepiece Okular Tube lens Objective Specimen

Primary image

$Refraction Kromatisk abberation$

Refraction Kromatisk abberation

Spherical abberation

Corrections The objective

Numerical aperture (NA) NA = nsina Resolution R = 0, 61 l/NA Rayleigh criterion (Raleigh in Brandon and Kaplan)

$The limiting factor: Diffraction NA = nsina Oil immersion$

The limiting factor: Diffraction NA = nsina Oil immersion

More is needed than just the objective Condenser system Eyepice Ocular (okular)

Optical anisotropy Calcite

$Amorphous and cubic: Optically isotropic, one refracive index Hexagonal, trigonal, tetragonal: Optically anisotropic, two$

Amorphous and cubic: Optically isotropic, one refracive index Hexagonal, trigonal, tetragonal: Optically anisotropic, two refractive indices Lower symmetry: Optically anisotropic, three refractive indices

Optically active crystals in the microscope

Michel-Levy chart

Remember that geologists often call their book on microscopy on thin sections for optical crystallography, and microscopy with reflected light is ore microscopy (malmmikroskopi). Some web sites: http: //www. olympusmicro. com/primer/ http: //www. microscopyu. com/ http: //www. micro. magnet. fsu. edu/primer/pdfs/basicsandbeyond. pdf maintenance: http: //www. zeiss. de/C 1256 D 18002 CC 306/0/1205 E 8 CBD 68054 EAC 125701500404705/$file/46 -0009_e. pdf