Medical School Histology Basics Blood and Lymph Vessels

Medical School Histology Basics Blood and Lymph Vessels VIBS 243 lab

Introduction Multicellular Organisms Need 3 Mechanisms ------------------------------1. Distribute oxygen, nutrients, and hormones 2. Collect waste 3. Transport waste to excretory organs

Multicellular Organism Needs met by cardiovascular system -------------------------------Distribute, Collection, and Transport are the functions of the Cardiovascular system Today we want to examine: How does variation in histological characteristics of different vessels facilitates their contributions to these functions? How does variation in histological characteristics of lymph vessels facilitates their contributions to these functions? CARDIOVASCULAR SYSTEM

CARDIOVASCULAR SYSTEM HEART PRODUCES BLOOD PRESSURE (SYSTOLE) Body Lungs Ref code # 10, 16, 19 Pressure seen along the arterial pathway from a single heart beat Pulse seen as sudden change in pressure and velocity is seen only in the arterial pathway = no pulse in capillaries and venous pathway but steady flow Velocity seen along the arterial pathway from a single heart beat Two sets of closed vessels connected only at the heart

CARDIOVASCULAR SYSTEM Ref code # 10, 16, 19 HEART contraction produces blood pressure during systole but contraction smooth muscle in the walls of other blood vessels reduces blood flow by reducing the caliber of the vessel lumen Variation in walls of blood vessels accommodates mechanical factors and metabolic needs Vessels vary in size, wall thickness, and shape and size of lumen to facilitate their functions

CARDIOVASCULAR SYSTEM ELASTIC ARTERIES - CONDUCT BLOOD AND MAINTAIN PRESSURE DURING DIASTOLE Ref code # 10, 16,

CARDIOVASCULAR SYSTEM Ref code # 6, 19 MUSCULAR ARTERIES DISTRIBUTE BLOOD, MAINTAIN PRESSURE ARTERIOLES - PERIPHERAL RESISTANCE AND DISTRIBUTE BLOOD CAPILLARIES - EXCHANGE NUTRIENTS AND WASTE VENULES - COLLECT BLOOD FROM CAPILLARIES (EDEMA)

Ref code #9

LAYERS IN VASCULAR WALL LAYER TUNICA INTIMA COMPOSITION ENDOTHELIUM (SUBENDOTHELIA CT. INTERNAL ELASTIC LAMINA) TUNICA MEDIA SMOOTH MUSCLE (ELASTIC LAMELLAE, EXTERNAL ELASTIC LAMINA) TUNICA ADVENTITIA CONNECTIVE TISSUE (LONGITUDINAL SMOOTH MUSCLE, VASA VASORUM) Ref code #6

MUSCULAR artery Ref code #9 34460 Large vein

Note flattened endothelial cells viewed on their side and on face (flat side of pancake) in arterioles in the iris (left side near the trabecular meshwork of the monkey eye Slide 34412 Iris of monkey eye Anterior chamber Arteriole Fattened endothelial cells On-face view Dilator muscle

• Slides 226, note that the endocardium Myocardium Endothelium Edocardium Connective tissue Layer of endothelium resting on a thin layer of connective tissue and covers the entire inner surface of the myocardium.

Epicardium 226 Myocardium 225 Fat

Slide HISTO 023 Heart muscle Myocardium Purkinje Fibers

Slide HISTO 024 Myocardium Endocardium Myocardium Heart valves Connective tissue

Slide HISTO 024 Connective tissue Myocardium Endothelium Endocardium Heart valve Connective tissue Endothelium

Aorta large elastic artery Slide 427 TUNICA ADVENTITIA TUNICA INTIMA TUNICA MEDIA Elastic laminae interspersed with smooth muscle cells Vasa vasorum TUNICA ADVENTITIA TUNICA MEDIA

Slide 427 Aorta TUNICA INTIMA Smooth muscle cells TUNICA MEDIA Elastic laminae

432 Lung elastic artery Elastic laminae TUNICA ADVENTITIA Fibroblasts TUNICA INTIMA TUNICA MEDIA Smooth muscle cells Vasa vasorum Endothelium

225 Heart Vasa vasorum Epicardium Myocardium Endocardium

34459 Muscular arteries in UT 196 B 1 Human spermatic cord Large vein Thick muscular tunica media Muscular arteries Wrinkled lumen Elastic membranes

472 Fallopian tube Elastic membranes Small vein venule Wrinkled lumen Muscular artery tunica media:

472 Fallopian tube muscular arteries Elastic membranes Muscular arteries tunica intima Tunica media Tunica adventitia Wrinkled lumen

126 Bile duct with portal vein, monkey Hepatic portal vein Tunica intima Tunica media tunica adventitia containing longitudinal muscle fibers

126 Bile duct with portal vein, monkey tunica adventitia containing longitudinal muscle fibers Common bile duct Tunica media Common bile duct Hepatic portal vein Endothelium

126 Bile duct with portal vein, monkey Common bile duct Tunica media Hepatic portal vein Endothelium tunica adventitia containing longitudinal muscle fibers

225 Heart myocardium Smooth muscle cells of an arteriole Autoregulation occurs by relaxation of smooth muscle cells in the walls of arterioles if local basal lamina O 2 levels are too low; thereby, increasing their caliber that increases local blood flow rate and increases O 2 levels in that locale.

Bile duct with portal vein, monkey 126 Arterioles Muscular artery Medium vein Fat cells Capillary Venules Muscular artery

472 Fallopian tube Arterioles Small vein Small artery Capillary Venules Muscular artery

EM 9 of arteriole: 1. Endothelial cell 2. Internal elastic membrane 3. Smooth muscle cell

Ref code #9 basal lamina liver muscle/brain kidney/adrenal

Ref code # 19 CONTINUOUS FENESTRATED SINUSOIDAL

TYPES OF CAPILLARIES & BASAL LAMINA CHARACTERISTICS CAPILLARIES LOCATIONS BASAL LAMINA CONTINUOUS COMPLETE MUSCLE, TESTIS, BRAIN, THYMUS FENESTRATED COMPLETE GLOMERULUS, ADRENAL DISCONTINUOUS OR SINUSOIDAL INCOMPLETE OR LACKING LIVER, SPLEEN, BONE MARROW MUSCLE GLOMERULUS EXAMPLES OF LIVER

186 Adrenal -cortex and medulla FENESTRATED Capillaries these are sinusoid blood vessels

155 Gall bladder & liver, monkey SINUSOIDAL capillaries

420 Rib bone marrow SINUSOIDAL capillaries in bone marrow

420 Bone marrow SINUSOIDAL capillaries

EM 10 a: pericyte in vessel wall; 20, 000 x 1. Lumen 2. Endothelial cell 3. Tight junction

EM 17 of capillary in the lamina propria of the intestine 1. Smooth muscle cell 2. Capillary 3. Endothelial cell

EM 10 b

136 Tongue Continuous capillaries in skeletal muscle CONTINUOUS capillaries in muscle

Histo 052 Tongue Continuous capillaries in skeletal muscle

32409 Small intestinal villi in the rat Lymphatic capillaries

126 Bile duct with portal vein, monkey Lymphatic capillary Muscular artery Lymphatic vessels identified by their lymph in the lumen of each Valves Lymphatic vessel with valves The lumen of blood vessels was cleared by vascular perfusion with fixative

LYMPH VESSELS FUNCTIONS RETURN PROTEIN, FLUID, AND BLOOD CELLS TRANSPORT SECRETIONS (HORMONES, ANTIBODIES) TRANSPORT FAT (NEUTRAL FAT) Ref code # 12

128 Thoracic duct tunica media Vasa vasorum of the tunica adventitia Circular and oblique smooth muscle bundles separated by connective tissue in the tunica media (the thickest layer) Smooth muscle

UT 196 valves in large vein of spermatic cord Valves Valve = connective tissue core draped with endothelium

196 UT 196 valve in lymphatic vessel in spermatic cord Lymphatic vessels Vein Valves in vessels Artery

In summary Ref code # 12, 19 Blood pressure generated Exchange in capillaries Fluid return via lymph Vessels adapted for mechanical and metabolic needs

Many illustrations in these VIBS Histology You. Tube videos were modified from the following books and sources: Many thanks to original sources! 1. Alberts, et al. , 1989. Molecular Biology of the Cell. 2 nd Edition. Garland Publishing, Inc. New York. ISBN 0 -8240 -3695 -6. 2. Alberts, et al. , 1994. Molecular Biology of the Cell. 3 nd Edition. Garland Publishing, Inc. New York. ISBN 0 -8153 -1619 -4. 3. Bloom, W. and Fawcett, D. W. , 1968. A Textbook of Histology. 9 th Edition. W. B. Saunders Company. Philadelphia. Library of Congress #67 -17445. 4. Elias, H. et al. , 1978. Histology and Human Microanatomy. A Wiley Medical Publication. John Wiley & Sons, New York. ISBN 0 -47104929 -8. 5. Eroschenko, V. 2000. Atlas of Histology with Functional Correlations. 9 th Edition. Lippincott Williams & Wilkins. Philadelphia. ISBN 07817 -2676 -X. 6. Fawcett, D. W. , 1986. Bloom and Fawcett. A Textbook of Histology. 11 th Edition. W. B. Saunders Company. Philadelphia. ISBN 0 -72161729 -8. 7. Fawcett, D. W. , 1986. Bloom and Fawcett. A Textbook of Histology. 12 th Edition. Chapman and Hall. New York. ISBN 0 -412 -04691 -1. 8. Guyton, A. C. 1971. Textbook of Medical Physiology. 4 th Edition. W. B. Saunders Company. Philadelphia. Library of Congress # 74118589. 9. Ham, A. W. 1974. Histology. 7 th Edition. J. B. Lippincott Company. Philadelphia. ISBN 0 -397 -52062 -X. 10. Ham, A. W. and Cormack, D. H. 1979. Histology. 8 th Edition. J. B. Lippincott Co. Philadelphia. ISBN 0 -397 -52089 -1. 11. Junqueria, et al. , 1995. Basic Histology. 8 th Edition. Appleton and Lange. Norwalk, Connecticut. ISBN 08385 -0567 -8. 12. Junqueira, et al. , 1998. Basic Histology. 9 th Edition. Appleton and Lange. Stamford, Connecticut. ISBN 0 -8385 -0590 -2. 13. Knobil, E. et al. 1988. The Physiology of Reproduction. Volume 1. Raven Press. New York. ISBN 0 -88167 -281 -5. 14. Langley, et al. , 1974. Dynamic Anatomy and Physiology. 4 th Edition. Mc. Graw-Hill Book Company. New York. ISBN 0 -07 -036274 -2. 15. Mescher, A. L. , 2010. Junqueira’s Basic Histology Text and Atlas. 12 th Edition. Mc. Graw Hill Medical. New York. ISBN 978 -0 -07 -1604314. 16. Tuttle, W. W. and Schottelius, B. A. 1969. Textbook of Physiology. 16 th Edition. The C. V. Mosby Company. Saint Louis. Library of Congress # 7589848. 17. Varner, D. et al. 1991. Diseases and Management of Breeding Stallions. American Veterinary Publications. Goleta, California. ISBN 0939674 -33 -5. 18. Von Hagens, Gunther and A. Whalley, 2007. Body Worlds – The Anatomical Exhibition of Real Human Bodies. ISBN 978 -3 -937256 -04 -7 19. Weiss, L. 1983. Histology: Cell and Tissue Biology. 5 th Edition. Elsevier Biomedical. New York. ISBN 0 -444 -00716 -4. 20. Weiss, L. and Greep, R. 1977. Histology. 4 th Edition. Mc. Graw-Hill Book Company. New York. ISBN 0 -07 -069091 -X.

Questions on blood vessels 1. Which of the following structure – function about the cardiovascular system do not match? a. Heart- produce blood pressure (diastole) b. Elastic arteries- conduct blood and maintain pressure during systole c. Muscular arteries- peripheral resistance d. a and b e. a, b, and c 2. Which type of capillary - location do not match: a. continuous - muscle b. fenestrated - glomerulus c. continuous – bone marrow d. a and b e. a, b, and c

Fossils in wall of Santa Elena Canyon – Big Bend National Park

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