Medical Microbiology Department of Microbiology HMU Department of
Medical Microbiology Department of Microbiology, HMU Department of Microbiology, Harbin Medical University
布鲁菌属 Brucella 引起人类、家畜和其它动物布鲁菌病 n 分6个生物种 n u 羊布鲁菌 u 牛布鲁菌 u 猪布鲁菌 u 鼠布鲁菌 u 绵羊布鲁菌 u 犬布鲁菌 B. melitensis B. abortus B. suis B. meotomae B. ovis B. canis Department of Microbiology, Harbin Medical University
生物学性状 n n 革兰阴性小杆菌 无鞭毛,不形成芽 胞,光滑型菌株有 微荚膜 Department of Microbiology, Harbin Medical University
专性需氧 n 生长缓慢 n 分解葡萄糖,产生少量酸 n 过氧化氢酶阳性,氧化酶阳性 n 产生H 2 S n B. melitensis Department of Microbiology, Harbin Medical University
流行环节 Department of Microbiology, Harbin Medical University
致病性 n 致病物质 u 内毒素、透明质酸酶、荚膜 Department of Microbiology, Harbin Medical University
所致疾病 n 母畜流产 u 赤藓醇--胎盘、绒毛膜含量高 Department of Microbiology, Harbin Medical University
S u m m a r y Brucella species
Biology Small gram-negative rods without capsule n Obligatory aerobic n Grows slowly in enriched culture media with 10% CO 2 n H 2 S + n Department of Microbiology, Harbin Medical University
Pathogens Human pathogen 1. 2. 3. Animal reservoirs Brucella melitensis Brucella abortus Brucella suis goat, sheep cattle pig Department of Microbiology, Harbin Medical University
Transmission n Enter human body by u Ingestion of contaminated milk products u Through the skin directly contact in an occupational setting such as abattoir Department of Microbiology, Harbin Medical University
Pathogenesis Brucella localized in the reticuloendothelial system (lymph nodes, liver, spleen, and bone marrow) n Cause granulomatosis, abscess, and caseation n No exotoxin is produced, so endotoxin is the major virulent factor n Department of Microbiology, Harbin Medical University
Disease: Brucellosis n Animal u Abortion n Human u Weakness and fatigue u Enlarged lymph nodes, liver, and spleen u Undulant fever (rising-and-falling fever) F Occurs in a few patients Department of Microbiology, Harbin Medical University
Diagnosis n Isolation u Sample: n blood, bone marrow Serological examination u Agglutination test u Complement fixation test u Coombs test n Skin test Department of Microbiology, Harbin Medical University
Prevention & Therapy Pasteurization of milk n Immunization n u Animals u Occupational high-risk persons Slaughtering of infected animals n Treatment with tetracycline and penicillin n Department of Microbiology, Harbin Medical University
- Slides: 23