Mechanical Bioreactor Integrated Systems Amanda Castagnino Emily Adams

Mechanical Bioreactor Integrated Systems Amanda Castagnino Emily Adams Emily Wood Natalie Nold Simran Singh

Goals and Deliverables Update on machining Update on device movement Strain % Tolerances Discussion PDMS Material Testing Results Static Cell Culture Results/Images Risk Assessment Update Bill of Materials Update MSD II Project Plan Agenda

PHASE III GOALS 1. Achieve specific device movement. 2. Machine parts for device mount. 3. Complete static cell culture testing in PDMS chamber. 4. Complete material testing on heat-cured PDMS. PHASE III DELIVERABLES 1. Machined device pieces 2. Cell Culture Images 3. Updated Risk Assessment 4. Updated Bill of Materials 5. MSD II Project Plan

New Membrane Holder: Solving Sterility Issue

Update on Machining Timeline ● ● ● Submitted Parts 3/9 Miscommunication resolved 3/19 2 parts received by 3/27 Reached out to Mike, Debartolo, and Rick WIll have new time estimate 3/30

Machining Problem Tracking. . .

Identifying & Selecting Problem PSP 1 Analyzing Problem PSP 2 Generating Potential Solutions PSP 3 Selecting & Planning Solution PSP 4 Implementing Solution PSP 5 Evaluating Solution PSP 6 R 2 R 3 Y 4 Y 5 G 6 Rating Description R 1 Major Machining parts takes much longer than expected 5 hours put into machining with no parts completely finished Time Major Not enough trained Consult machine team members and Ask machine shop to shop and get time experience to make machine parts for us estimate designed parts open up 10 days since files unclear communication with submitted, only 2 parts communication with shop and reach out done machine shop to debartolo Connected with debartolo and Houdini Incorrect tapping tolerances Provided incorrect tapping tolerances to machine shop Lack of machining knowledge Sheet metal drawing have not completed draw for membrane holder do no know how to create sheet metal drawing delegate task to amanda, who will learn how complete drawing Pegged Plate Pegs are not perpendicular Pegs Tapped poorly Confront shop Remake part Arm Complicated piece to machine needs welding and press-fit Consult Houdini Mold Complicated piece to machine needs press-fit and high tolerances Consult Houdini determine correct edit and resubmit size of tapped holes files meeting with machine shop Friday files resubmitted Houdini is making part

Device Movement Update ● The wall power supply arrived last night. ● Working on making the actuator cycle through a test. ● Working on measuring the displacement of the actuator compared with the potentiometer slack.

Actuator Precision ● ● At the moment, the measurements have all been in 1 mm increments and anything less has been an estimate. This means 2. 5% range for each value. At best, the actuator can do 0. 1 mm increments. This means the smallest value for the range would be 0. 25%. With the current slack showing, we are getting a possible range of of 0. 3 mm to 0. 6 mm (0. 7% to 1. 4%). Things could change when we find a better way to measure the distance.

% Strain Tolerances The table shows values of the generalized values. Due to material properties and accuracy of actuator, these strain values are generalized and may not be the actual values. % Strain Stretch (mm) % Range w/ accuracy (using 1. 4%) 5 2 3. 6 - 6. 4 10 4 8. 6 - 11. 4 15 6 13. 6 - 16. 4 20 8 18. 6 - 21. 4 Varying thickness in PDMS chamber could lead to a greater proportion of stretching in the well of the chamber.

Material Testing with Heat-Cured PDMS “N” Control PDMS cured at 100°C for 35 minutes. Testing tab slipping reduced with heat-cured samples. “I” Incubated at 37°C three days before material testing

Results

Results

Results Sylgard 184 TDS: Tensile Strength (6. 7 MPa) Strains: Standard (58. 8%) - 43. 9% Worst Case Incubator (55. 5%) - 33. 1% Worst Case

Static Cell Culture Images 4 X Objective - Leica 10 X Objective - Leica 2. 5 X Objective - Leica

Risk Assessment Limited project time increases risk

Bill of Materials Machining and cell culture supplies purchased Total Budget Left ~ $350

MSD II Project Plan - Final Phase Finish machining parts and creating cycling programs Test experiment conditions Complete user documentation Test with subjects as much as possible

Project Deadlines April 19 Finished final poster Technical paper submitted for review April 24 Finished EDGE website Performance vs Requirements Lightning Talk Slide April 26 Technical Paper due April 27 Industrial Advisory Board Demo April 28 Imagine RIT Festival May 2 Workspace clean May 2 -4 Final Gate Review

Three-Week Plans Amanda Dynamic cell culture testing in PDMS chamber Device 3 -D printing and machining Emily A. Emily W. Finalize programs for device Finish machining device parts Verify % strain movement and tolerances Natalie Simran Finalize technical report Dynamic cell culture testing in PDMS chamber Create Imagine RIT poster Finalize device documentation Create guide for cell imaging and analysis