Mechanical Bioreactor Build and Test Prep Amanda Castagnino

Mechanical Bioreactor Build and Test Prep Amanda Castagnino Emily Adams Emily Wood Natalie Nold Simran Singh

Goals and Deliverables Updated Cell Chamber Design Updated Device Model Design Electrical Schematic Cell Analysis Update Problem-Tracking Document Risk Assessment Update Bill of Materials Update MSD II Project Plan Agenda

PHASE I GOALS 1. Create electrical schematics for device 2. Finalize CAD model of device 3. Create new iteration of chamber mold PHASE I DELIVERABLES 1. Updated PDMS mold of cell chamber. 2. Electrical Schematic 3. Complete CAD model of device 4. Problem-Tracking Document 5. Updated Risk Assessment 6. Updated Bill of Materials 7. MSD II Project Plan

Chamber Mold

CAD Models

CAD Models

CAD Models

Electrical Schematic

Electrical Schematic (cont. ) Arduino/LCD screen

Motor Controller

Cell Analysis Update ● ● Discussed with Dr. Abhyankar about how to use Image. J to analysis cells for experiment Changes in cell orientation can be analyzed Changes in cell size (length and width) can be analyze There are plugins that Dr. Abhyankar is developing in his lab to do some similar cell analysis that we may be able to use

Risk Assessment

Bill of Materials Boxed: Items recently ordered.

Problem-Tracking

MSD II Project Plan Next phase: Develop working prototype 3 -D print model Assemble prototype device Achieve device movement Strength testing for heat cured PDMS (once vacuum oven is functional) Imagine RIT Submission and final report draft

MSD II Project Plan Define user input for device movement Static cell culture testing and Image. J analysis Test experiment conditions and ease of use Dynamic cell culture testing with working device