MCB 151 S P R IN G 2

MCB 151 S P R IN G 2 0

COURSE INFORMATION MCB 151 Website: http: //www. life. uiuc. edu/mcb/151/ Login: Net. ID Password: AD password

Course Syllabus SP 20 Week of Laboratory Topic January 20 No labs this week MLK Jr Day – January 20 January 27 Week 1: Introduction February 3 Week 2: Bacteria February 3 - Last day to add course or change sections February 10 Week 3: Eukaryotic Cell February 17 Week 4: Enzymes February 24 Week 5: DNA March 2 Week 6: Gel Electrophoresis March 9 Week 7: Exam I (Exercises 1 -6) March 13 – Last Day to drop MCB 151 March 16 -20 No labs this week – Spring Break March 23 Week 8: UV Damage and Mutagenesis March 30 Week 9: Cell Cytoskeletal Structure April 6 Week 10: p. GLO Transformation April 13 Week 11: HIV ELISA April 20 Week 12: Exam II (Exercises 8 -11) April 27 Optional Review for Final Exam May 4 No labs this week May 6 – Last Day of Classes & Web Gradebook Deadline (5 PM) May 7 Reading Day TBA Final Exam (cumulative)

REQUIRED MATERIALS Required Texts and Supplies 1. "Laboratory Exercises for MCB 151: Molecular & Cellular Biology, Spring 2020" Melissa Reedy, editor Stipes Publishing 2. Biology Laboratory Notebook (carbonless) Stipes Publishing Recommended (Optional) Texts Textbook ISBN is: 0134243064. Biological Science (Freeman et. al, 7 th edition) - currently used in MCB 150

ADD, DROP, SECTION CHANGE • Students may use the UI–Integrate Self-Service System to add or change MCB 151 laboratory sections before 5: 00 PM, Monday, February 3, 2020. • Friday, march 13 is the last day to drop the course or to elect the Credit/No Credit option. Students may use the UI-Integrate Self. Service System to drop MCB 151 until this deadline.

COURSE GRADING (725 POINTS TOTAL) Laboratory Reports § 8 @ 75 points each, 525 points (drop 75 points) Laboratory Exams § 2 @ 50 points each Final Exam § 1 @ 100 points § *Only students that are present and on time are eligible to complete the Lab Report for that particular week. If you are absent/late, you are not eligible to complete the Lab Report for that particular week and you may not be eligible to submit the previous weeks Lab Report.

READING ASSIGNMENTS The Reading Assignment for each week can be found on the course web site under the Laboratory Exercises link. These readings supplemental, all material will come from the lab manual and lab experience.

http: //www. life. illinois. edu/mcb/151/

LON-CAPA • LON-CAPA will be used for your exams in this course. Instructions for accessing LON-CAPA can be found on pages I-17 and I-18. • Exams 1 & 2 will be administered in your lab section the week of March 9 and April 20. • The Final Exam will take place during finals week, date/time/location TBA.

LON-CAPA

HTTP: //POLICE. ILLINOIS. EDU/EMERGENCYPLANNING/ In an emergency in this building, we’ll have three choices: RUN (get out), HIDE (find a safe place to stay inside), or FIGHT (with anything available to increase our odds for survival). First, take a few minutes this week and learn the different ways to leave this building. If there’s ever a fire alarm or something like that, you’ll know how to get out, and you’ll be able to help others get out too. Second, if there’s severe weather and leaving isn’t a good option, go to a low level in the middle of the building, away from windows. If there’s a security threat, such as an active shooter, we’ll RUN out of the building if we can do it safely or we will HIDE by finding a safe place where threat cannot see us. We will lock or barricade the door and we will be as quiet as possible, which includes placing our cell phones on silent. We will not leave our area of safety until we receive an Illini-Alert that advises us it is safe to do so. If we cannot run out of the building safely or we cannot find a place to hide, we must be prepared to fight with anything we have available in order to survive. Remember, RUN away or HIDE if you can, FIGHT if you have no other option. Finally, if you sign up for emergency text messages at emergency. illinois. edu, you’ll receive information from the police and administration during these types of situations. If you have any questions, go to police. illinois. edu, or call 217 -333 -1216.

EMERGENCIES IN THE LAB • Eye Wash • Fire Extinguishers • Fire Alarms • First Aid

COURSE COORDINATOR Melissa Reedy Course Coordinator MCB 150/151 Office: 415 Burrill Hall Phone: 265 -6379 Email: murray@illinois. edu Office hours: By appointment

EXERCISE 1 IN T L R A O D T B B U A S E C C IC T H IO N S K N : IQ IL U L E S S A N D S A F E T Y

LABORATORY SAFETY Bacteria: § Wear gloves § Wash you hands before leaving the lab § Notify your TA of any spills or contamination Chemicals: § Always handle reagents with caution UV Light: § Never look at UV lamps § Wear protective gloves

LABORATORY TECHNIQUES Clean up: each student is responsible for cleaning up after every experiment Labels: always label your experimental work with your name and section, b/c we share incubators, refrigerators, water baths, etc. Pipetting: always review the proper use of pipettes before beginning experiments Centrifuges: used to spin down and separate

LAB SAFETY IMPORTANCE Work you are doing now can have consequences now and in the future § Toxic or infectious substances could result in acute or chronic illness or even death. § Explosions or fires can injure or kill. Knowledge of safety is critical in protecting yourself and others from potential hazards.

HOUSEKEEPING Bench tops, floors and other surfaces must be kept clean and free of clutter § Coats and backpacks must be hung on coat hooks in designated areas To avoid accidental ingestion of toxic or infectious materials, the following are prohibited in the lab: § Eating or drinking § Taking medication § Applying lip balm, gloss, lipstick or make-up

HAND HYGIENE Wear gloves in the lab when directed to do so § Make sure to choose gloves that are the appropriate size and fit well § Do not use gloves that have holes or cracks § Always remove gloves before leaving the room § Remove gloves by pulling cuff down over your hand, turning the glove inside out § Never touch door knobs or handles with gloved hands Always wash hands after removing gloves

PERSONAL PROTECTIVE EQUIPMENT Students may elect to wear safety glasses or goggles, which are recommended but not required Students may also elect to wear lab coats PPE can be purchased from: § Life Sciences storeroom, C 107 CLSL, phone: 217 -333 -7693 § RAL storeroom, 94 RAL, phone: 217 -333 -3564

PERSONAL ATTIRE IN THE LAB The following guidelines apply to attire in the lab: § Clothing should cover legs and midriff § Footwear should completely cover feet (no open-toed shoes or sandals) § Long hair should be tied back § Loose sleeves or tops should be confined

BIOSAFETY LEVELS Biosafety is the application of a combination of laboratory practices and procedures, laboratory facilities, and safety equipment when working with biological material Biosafety levels: § BL 1 - Agents not known to cause disease consistently in healthy humans § BL 2 - Agents associated with human or animal disease § BL 3 - Indigenous/exotic agents associated with human or animal disease and with potential for aerosol transmission § BL 4 - Dangerous/exotic agents of life threatening nature

SHARPS The Illinois EPA defines sharps as: § Any medical needle § Syringe, with or without needle § Scalpel and razor blade § Blood vials § Pasteur pipets § Microscope slides and coverslips § Glassware contaminated with infectious agents Sharps must be disposed of in approved Sharps container

BIOLOGICAL WASTE Cultures, stocks and disposable labware generated from experiments with biological materials must be autoclaved prior to disposal § Place these materials, as directed, into red biological waste disposal bags in the lab § Do NOT put these materials into regular trash § If you are uncertain, please ask!

EMERGENCY PREPAREDNESS Please note the location of the following: § Shower § Eyewash § Fire extinguisher § Spill kit § Nearest exit If you must, evacuate to prevent or minimize injury

FIRE SAFETY If fire occurs: § Notify those in the immediate area § Activate nearest fire alarm § Confine the fire by closing doors and windows § Attempt to extinguish the fire only if it is safe to do so § Do not use elevators § If possible, call 911 to explain the nature of the fire or emergency and meet responders outside

DRS The DRS website provides campus-specific safety information such as: § Safety guidance documents in the "Safety Library” § Online Safety Trainings § Information on how to manage hazardous waste § Information on DRS safety programs § Accident response information § Templates and forms for managing hazards in the laboratory

, EXERCISE 1 B: BASIC LABORATORY SKILLS

GOALS • • • Understand manipulate exponential numbers. Become familiar with the units used for small concentrations and volumes and be able to make conversions between them. Figure out concentrations of solutions or cell suspensions after dilution. Understand what is meant by p. H and know how to calculate the p. H of a solution. Understand how buffers work.

EXPONENTIAL NUMBERS • Every number can be expressed as the product of two numbers, the second one being some power of ten. § For example, the number 100 can be expressed as 10 X 10 = 102 § The same rules are used to express numbers less than one. 10 -2 = 1/10 X 1/10 = 1/100

UNITS OF MEASUREMENT Common units Mass Volume 1 gram (g) = 103 milligrams (mg) = 106 micrograms (µg) = 109 nanograms (ng) 1 liter (l) = 103 milliliters (ml) = 106 microliters (µl) = 1 cubic centimeter 1 ml Length 1 meter (m) = 103 millimeters (mm) = 106 micrometers (µm) = 109 nanometers (nm)

DILUTIONS C 1 V 1 = C 2 V 2 Example: Given a 1 M stock solution of Tris buffer prepare 10 ml of 0. 01 M Tris. Solution: The question you need to answer is "what volume of 1 M Tris do you need to dilute to give a total volume of 10 ml of 0. 01 M Tris". Thus, you know C 1, C 2, and V 2. (? ml x 1 M) = (10 ml x 0. 01 M) ? ml = 10 ml x 0. 01 M (1 M) Answer: 0. 1 ml

DILUTION FACTOR Dilution = 1/100 Dilution Factor: 100 Dilution = 1/n Dilution Factor: n Serial Dilution: product of the individual dilutions 1/102 x 1/103 = 1/106 = 10 -6

AQUEOUS SOLUTIONS An aqueous solution is one in which water is the solvent. Water properties: High surface tension High boiling point Good Solvent

ACIDS, BASES, PH AND BUFFER Acids/Bases: Bronsted Definition: an acid is a molecule that donates protons and a base is a molecule that accepts protons p. H: Dependent upon the concentrations of H+ and OH- ions Buffers: A molecule that allows the solutions to resist changes in the hydrogen ion concentration

EXERCISE 1 C: MICROSCOPY

GOALS/TECHNIQUES • Develop working knowledge of a brightfield microscope. • Discern between different types of microscopy. • Practice techniques Pages 24 -35 in your manuals

ASSIGNMENTS FOR NEXT WEEK • Reminder: No labs next week, labs resume September 9 • You must come to the next lab with your pre lab write up complete for Exercise 2 Parts 1 and 2 • Purpose • Procedure/Protocol