Marimo Bacteria Intro Design Marimo Overview signal Marimo

Marimo Bacteria Intro ここの内容は暫定版です Design Marimo? Overview signal Marimo is known as a spherical shaped algae which could be found, for example, in the Lake Akan in Hokkaido, Japan. It is such a popular living organism in our country as a National Treasure. When you come to Japan, you absolutely come across to Marimo in aquariums, TV programs or Mangas : ) Why Marimo? sticked signal sticked Sender Core Receiver Culture signal Sender Core Receiver Culture 1. Make a sender and … 2. 3. … この部分はもういちどプレゼンふまえて考えます． Requirements for Our System When you see a shape in Nature, you will notice whether a sphere, which is absolutely symmetric in 3 D, is really stable or not in Nature. multicellular organisms have their shape different from a sphere except Marimo. Of course, other algae do not show spherical shape, they live on a surface of stone. It is quite intriguing how Marimo remains its spherical shape in a lake! How Marimo? 1. Sticky Tag Design(Aim? Purpose? ): Insert his-tag into flagella Background: 2. Communication Module 3. Size Controler Design: Make a logical circuit to localize AHL. Three receiver circuit was designed. Design: Improve senders & receivers to communicate Background: fli. C Mutating Lux. R (used in quorum sensing) leads to a sensitive AHL detector. Fli. C is the E. coli flagellar fillament protein. The D 3 domain in fli. C accommodates the insertion of peptides. (ref) Background: aii. A, the AHL quencher. (ref) Experiments & Results 1. Sticky Tag signal i) Making Affinity Tag Swarm Phenotype Achivements /Future Inserted his-tag(6 histidine residue) into fli. C D 3 domain. ii) Affinity Check Method: Beads adsorption. Beads with metal ions will bind with his-tag >GI 826(fli. C mot. B) showed affinity result Fig 1. Beads adsorption in strain JW 1908(fli. C) result Fig 2. Beads adsorption in strain GI 826(fli. C mot. B) New Parts 2. Communication Module i) Improving Sender Overexpressed the met. K (the AHL precursur synthesis enzyme) in the hope to increase AHL synthesis. > Didn’t work. Lux. I seems to be the rate-determing step of AHL synthesis. ? ii) Sensitizing Reciever Inserted 2 mutations in Lux. R which is known from the paper to increase activity. > Didn’t work. Needed additional exp. 3. Size Controller すみませんここはまだ考え中です… Conclusion