Mapping leaf rust resistance genes in wheat Jim

Mapping leaf rust resistance genes in wheat Jim Kolmer USDA-ARS Cereal Disease Laboratory Jim Anderson Univ. of Minnesota Thanks to Kun Xiao, Jen Flor

RL 6149 = Thatcher*6/T. turgidum 8404 Developed by P. Dyck RL 6149 IT is ; (fleck) 20 -50 MRMS Thatcher x RL 6149 F 2 (: - 3) 100 (3+4) 30 F 3 families 32 HR 63 Seg 25 HS

RL 6149 F 3 families used for mapping 6 AL location – flanked by Barc 104 (13. 9 cm) and GWM 427 (21 cm) No other Lr gene on 6 AL Designated as Lr 64

RL 6062 RL 6020 = Thatcher*6/ PI 268316 Developed by P. Dyck PI 268316 common wheat RL 6062 22+ IT 30 -50 MRMS Thatcher x RL 6062 F 2 (2 -2+ ) 90 (3 -4) 43 F 3 families 26 HR 73 SEG 36 HS Infection type F 2

RL 6062 Polymorphic SSR markers not linked to resistance gene Bulk Segregation Analysis 15 HR 15 HS F 3 families 8 DAr. T markers on 6 BL in disequilibrium – distal region SSR data Lr 3 Lr 9 – linkage? RL 6062 isolate specificity different P. L. Dyck - 1980

Americano 25 e Uruguay landrace derived 1918 – highly resistant Tc*2/Americano 25 e F 6 Adult plant resistance – no seedling resistance 10 -20 MRMS field resistance 0; 2 - adult plant IT greenhouse test Tc*3/ Americano 25 e F 6 lines Three field tests – greenhouse test 1 -2 genes segregating Severity (%)

Tc*3/Americano 25 e F 6 lines DAr. T map Major effect on 1 BL – likely Lr 46 Select Tc*3 lines with Lr 46 only

Tc*3/Americano 25 e F 6 lines Smaller effect on 5 B No effect St Paul 2010 (fewer high severity lines) Select Tc*3 lines with 5 B QTL

Tc*3/Americano 25 e F 6 lines R 2 values Position St. P 2009 St. P 2010 Crk 2010 GH 1 BL 26 0. 254 0. 293 0. 392 0. 379 28 (Lr 46 mark) 0. 257 0. 303 0. 382 0. 409 30 0. 263 0. 299 0. 381 0. 411 32 0. 265 0. 292 0. 373 0. 408 34 0. 264 0. 281 0. 362 0. 399 36 0. 259 0. 266 0. 346 0. 386 38 0. 251 0. 248 0. 325 0. 367 50 0. 137 0. 007 0. 144 0. 112 52 0. 141 0. 008 0. 155 0. 117 54 0. 138 0. 009 0. 160 0. 120 56 0. 124 0. 009 0. 155 0. 118 5 B