Male infertility Male infertility Reproductive medicine has undergone

남성 불임증 Male infertility

남성불임 Male infertility Reproductive medicine has undergone tremendous changes during the 1990 s. assisted reproductive technique (ART) With the success of these high-technologic, high-cost procedures, the evaluation of the male is often bypassed. This approach ignores the fact that many causes of male infertility, such as varicocele, ductal obstruction, and infections, are easily and effectively treated. In addition, without a full evaluation, significant diseases, such as testicular cancer, pituitary tumors, and neurologic disorders, may be overlooked

chance of a normal couple conceiving is estimated to be 20% to 25% per month, 75% by 6 months, and 90% by 1 year. approximately 15% of couples are unable to do so. Most conceptions occur when intercourse takes place within 6 days before and including the day of ovulation. Approximately 20% of cases of infertility are caused entirely by a male factor, with an additional 30% to 40% of cases involving both male and female factors. Therefore, a male factor is present in one half of infertile couples.

I. Causes of male infertility 1. Pre-testicular causes 1) Isolated gonadotropin deficiency (Kallmann's syndrome) familiar form; inherited either autosomal recessive or dominant associated with anosmia I. except gonadotropin deficiency, anterior pituitary function intact II. other congenital anomaly; congenital deafness, harelip, cleft palate III. craniofacial symmetry, renal abnormality IV. 2) Pituitary disease VI. prolactinemia, hemochromatosis VIII. 3) Exogenous or endogenous hormone IX. estrogen/androgen excess, glucocorticoid excess, X. hyper- and hypothyroidism XI.

2. Testicular causes 1) Chromosomal abnormality (1) Klinefelter's syndrome; presence of extra X chromosome 10 % of patient have mosaicism; less severe feature and may be fertile small, firm testis, decreased androgenecity, azoospermia, gynecomastia Leydig cell may appear hyperplastic, normal number gonadotropin level are elevated, particularly FSH normal or decreased testosterone level, elevated estradiol in serum (2) XX disorder (sex reversal syndrome) 2) Bilateral anorchia (vanishing testis syndrome) 3) Sertoli cell only syndrome (Germinal cell aplasia) congenital absence of germ cell, genetic defect, androgen resistance testicular biopsy reveal complete absence of germinal element azoospermia with normal virilization normal consistency but slightly smaller size, no gynecomastia normal LH, testosterone and elevated FSH

3. Post-testicular causes 1) Disorders of sperm transport 2) Disorders of sperm motility or function (1) congenital defects of sperm tail immotile-cilia syndrome Kartagener's syndrome; immotile sperm, chronic sinusitis, bronchiectasis, situs inversus, (2) maturation defect (3) immunologic defect

II. Clinical findings 1. History male reproductive history; duration of unprotected coitus, previous pregnancy female reproductive history: age, gravida/para, ovulation, corpusluteal function personal history; puberty, undescended testis, surgical, gonadotxin, sexual, medication family history; hypogonadism, cystic fibrosis endocrine history; most experts advise vaginal intercourse every 2 days, which ensures that viable sperm are present during the 12 - to 24 -hour period in which the oocyte is within the fallopian tube and is capable of being fertilized. after a febrile illness, spermatogenesis may be impaired for 1 to 3 months. in patients with abnormal semen analyses and a history of a systemic illness within 3 months of the evaluation, additional semen analysis should be obtained over a 3 - to 6 -month period to adequately assess the patient's baseline fertility status.

2. Physical examination 1) Eunuchoid skeletal proportion Upper-body: lower-body ratio <1 arm span 2 inches greater than height 2) Lack of adult male hair distribution sparse axillary, pubic, facial, body hair lack of recession of hair on temporal bone 3) Infantile genitalia small penis, testes, and prostate, underdeveloped scrotum 4) diminished muscular development and mass

3. Laboratory studies 1) Semen analysis highly predictive of significance of male factor specimen collected in clean, wide mouthed container or in special condom should be examined within 2 hours standard period of abstinence is 2 - 3 days azoospermia: qualitative test for fructose (1) Low ejaculate volume, lack of fructose, failure of coagulation: a. seminal vesicle absence b. absence of vas deferens c. atresia ejaculatory duct

(2) Minimal standards of adequacy (WHO, 1999) volume p. H sperm concentration total sperm number motility morphology viability WBC >2. 0 m. L >7. 2 > 20 million sperm/ml > 40 million sperm/ejaculation > 50% with grade A+B > 25% with grade A > 15% by strict criteria > 75 % < 1 million/ml although may be used to determine normal from abnormal, does not separate fertile from infertile men.

(3) Computer-assisted semen analysis(CASA) new technique for semen analysis VCL (curvilinear velocity): average distance per unit time between successive position sperm along its path VSL (straight line velocity): distance between first and last position on path divided by total elapsed time LIN (linearity): VSL/VCL, measure of straightness of swimming ALH (amplitude of lateral head displacement): average perpendicular distance of lateral position of sperm head with respect to average swimming path currently, CASA is not to give a more accurate prognosis or to affect treatment as compared with a manual semen analysis

(4) Retrograde ejaculation confirm by reduced semen volume and presence of many sperm in post ejaculation urine specimen. a. TUR or open surgical resection of bladder or prostate b. bilateral sympathectomy c. bilateral retroperitoneal lymphadenectomy d. extensive pelvic surgery; proctectomy and colectomy e. diabetic visceral neuropathy f. antihypertensive drug; block sympathetic tone

2) Endocrine test primary endocrine defect in infertile man is less than 3 % (1) baseline hormonal evaluation LH, testosterone , FSH distinguish primary testicular failure (hypergonadotrophic hypogonadism) from hypogonadotrophic hypogonadism

2) dynamic hormonal testing single pulse dose is inadequate a. h. CG test b. Gn. RH test differentiation hypogonadotrophic hypogonadism or pituitary origin from hypothalamus origin 500 mg of Gn. RH given daily for 3 days serum LH should be measured before and 60 min after last Gn. RH 3) Chromosomal study

4) Immunologic study anti-sperm antibody is in 3 -7% of infertile man history of inflammation, testis injury torsion, vasectomy may disrupt blood testis barrier Risk factors of antisperm antibody impaired sperm motility sperm agglutination abnormal PCT (post coital test) finding couple with unexplained infertility

5) Testicular biopsy: azoospermia with normal FSH level

Seminal vesiculography, Vasography to determined site of obstruction in azoospermic patient, have active spermatogenesis by testis biopsy

6) Sperm function test (1) postcoital test (PCT) (2) sperm penetration assay (3) hemizona assay (4) hyposmotic swelling test (HOS) (5) hyperactivity test

7) Bacteriologic investigation Chlamydia trachomatis Mycoplasma hominis Ureaplasma urealyticum 8) Androgen-receptor abnormality 9) Radiologic investigation (1) scrotal ultrasonography; varicocele (2) venography; varicocele (3) transrectal ultrasonography; identify obstruction or congenital anomaly of ejaculatory duct or seminal vesicle

Goals of evaluation of infertile male are to identify (1) reversible conditions; (2) irreversible causes that may be managed by ARTs using the male partner's sperm; (3) irreversible conditions that may not be managed by these techniques and in which the couple should be advised to pursue donor insemination or adoption; (4) significant underlying medical pathology; (5) genetic and/or chromosomal abnormalities that may affect either the patient or his offspring.

III. Differential diagnosis of male infertility 1. Treatable causes 1) varicocle 2) obstruction (acquired /congenital) 3) infection 4) ejaculatory dysfunction 5) hypogonadotrotic hypogonadism 6) immunologic problem 7) sexual dysfunction 8) hyperprolactinemia 2. Potentially treatable causes 1) idiopathic 2) cryptorchidism 3) vasal agenesis 4) gonadotoxin (drug, radiation) 3. Untreatable causes 1) bilateral anorchia 2) germinal cell aplasia 3) primary testicular failure 4) chromosomal anomaly 5) immotile cilia syndrome

IV. Treatment 1. Surgical measure 1) Varicocelectomy

2) Vasovasostomy 3) Epididymovasostomy

4) Transurethral resection of ejaculatory duct 5) microsurgical epididymal sperm aspiration (MESA) 6) prophylactic surgical measure (1) orchiopexy (2) operation of testicular torsion (3) electroejaculation

2. Medical measure 1) Endocrine therapy hypogonadotropic hypogonadism: h. CG, h. MG Gn. RH 2) Therapy for immunologic infertility corticosteroid 3) Therapy for retrograde ejaculation alpha sympathomimetic innervation 4) Treatment of infection 5) Empirical therapy a. antiestorgen; clomiphene b. historical therapy

3. New therapy (assisted reproductive techniques : ARTs) 1) semen processing; concentrated highly motile sperm in small volume 2) intrauterine insemination (IUI); by-pass cervix 3) In vitro fertilization (IVF) or in vitro fertilization/embryo transfer 4) gamete intrafallopian tube transfer (GIFT) 5) micromanipulation (1) zona drilling (2) partial zona dissection (PZD) (3) subzonal sperm injection (SZI, SUZI) microinsemination sperm transfer (MIST) (4) microinjection of sperm into ooplasm intracytoplasmic sperm injection (ICSI)