Maintained Properties of Aged Dental Pulp Stem Cells

Maintained Properties of Aged Dental Pulp Stem Cells for Superior Periodontal Tissue Regeneration Linsha Ma, Jingchao Hu, Yu Cao, Yilin Xie, Hua Wang, Zhipeng Fan, Chunmei Zhang, Jinsong Wang, Chu-Tse Wu, Songlin Wang 1 Molecular Laboratory for Gene Therapy &#x 00026; Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University, School of Stomatology, Beijing, China 2 Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing, China 3 Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Beijing, China Figure 5. More hard tissue regeneration in the periodontium after DPSCs injection compared to BMMSCs injection in vivo. (A-C) Gross micro-CT view of periodontal defect regeneration in the control group (saline injection), DPSC group, and BMMSC group. (D-F) Histopathological assessment of periodontal bone regeneration by HE staining in the periodontal defects of the DPSCs group and BMMSCs group. (D&#x 02019; -F&#x 02019; ) DOI: 10. 14336/AD. 2018. 0729 Impaired sulcular epithelia and lymphocyte infiltration were evident in the control group compared with the DPSCs and BMMSCs groups. (D&#x 02019; -F&#x 02019; , G, H) Alveolar bone heights in the DPSCs and BMMSCs groups were much larger than those of the control group. Values are means &#x 000 B 1; SDs. One-way ANOVA was used to determine statistical significance. Error bars represent SDs (n = 5). &#x 0002 A; P &#x 02264; 0. 05; &#x 0002 A; P &#x 02264; 0. 01. Scale bar: 100 &#x 003 BC; m.