Maintained Properties of Aged Dental Pulp Stem Cells

Maintained Properties of Aged Dental Pulp Stem Cells for Superior Periodontal Tissue Regeneration Ma Linsha, Hu Jingchao, Cao Yu, Xie Yilin, Wang Hua, Fan Zhipeng, Zhang Chunmei, Wang Jinsong, Wu Chu-Tse, Wang Songlin 1 Molecular Laboratory for Gene Therapy &#x 00026; Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University, School of Stomatology, Beijing, China 2 Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing, China 3 Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Beijing, China Figure 4. DPSCs show higher resistance to LPS-induced senescence and dysfunction of osteogenesis compared to BMMSCs ( A ) SA &#x 003 B 2; -gal staining of DPSCs and BMMSCs under TNF&#x 003 B 1; and LPS stimulation. Quantitative analysis indicated significantly more SA &#x 003 B 2; -gal-positive cells in BMMSCs compared to DPSCs under TNF&#x 003 B 1; and LPS stimulation. ( B ) Annexin V/PI staining and quantitative analysis showed lower rates Aging and Disease, 2019, 10(4), 793 -806. DOI: 10. 14336/AD. 2018. 0729 of cell apoptosis in DPSCs than BMMSCs under LPS and TNF&#x 003 B 1; stimulation. ( C ) Alizarin Red staining and quantitative measurements of osteogenesis of DPSCs and BMMSCs under LPS and TNF&#x 003 B 1; stimulation. ( D ) HE staining and quantitative measurements of the osteogenic property of DPSCs and BMMSCs in vivo under LPS and TNF&#x 003 B 1; stimulation. Values are means &#x 000 B 1; SDs. Student&#x 02019; s t-tests were used to determine statistical significance. Error bars represent SDs (n = 5). &#x 0002 A; P &#x 02264; 0. 05; &#x 0002 A; P &#x 02264; 0. 01; NS: no significance. Scale bar: 100 &#x 003 BC; m.