Lysine Methylation and Chromatin Biology Lab Dan Levy

Lysine Methylation and Chromatin Biology Lab Dan Levy Faculty of Health sciences, Ben-Gurion University Department of Microbiology, Immunology and Genetics me

me me PTMs: me me Methylation me Ph Phosphorylation Ac me CLOSED P Chromatin Dynamics TF me Ac Acetylation OPEN Many others. . . How PTM’s influence chromatin structural dynamics? me Gene expression programs in physiological and pathological processes Inflammatory disorders Cancer Methylation

Protein lysine methylation protein lysine methyltransferases >50 PKMTs H H N+ H PKMT PKDM Mono(me 1) me Lysine N+ PKMT PKDM e m H PKMT Di(me 2) me PKDM H N+ K K Function B Function C K Function A H >25 PKDMs Chromo domains Tudor domains PHD fingers PWWP domains Methyl-lysine binding modules “READERS” Tri(me 3) e m me m e N+ K Ankyrin repeats WD repeats protein lysine demethylases MBT domains Function D

How to identify a new methylation event? H 3 H 4 p 53 Rb H 4 H 2 B H 2 A ERa ? ? ? Histones Non-histones me me Candidate approach me ? Protein and peptide arrays High throughput approach

Biochemical Proteomics Genomics Cellular Methylation event Mechanisms Function Binders Cancer me New Substrates PKMT A B C D K Prot-Prot Interaction Cellular Methylation pattern me me me Differentiation Chromatin structure me Ac Ph ? ? ?

Thank you!