LOOKING AT CELLS History of the Microscope Types

Looking at Cells are VERY tiny! ________= ___________(_____) =approximate width of the average fingernail

What came first? The cell or the microscope? Anton van Leeuwenhoek’s microscope, established circa

_______________ • A Dutch scientist born in _______. • ------------------------------------------------------. • His new improved

______________ • Robert Hooke, an English scientist, was the first scientist ______________. • When

1700’s 1800’s 1600’s Today The origin of the microscope is a matter of debate.

________Microscope _____________: • The compound microscope has __________________________. • This microscope is ideal for

_____________ An electron microscope is any microscope __________________________ of a specimen. However, they are

Transmission Electron Microscope (TEM) • Original electron microscope • Invented in the ____. •

Scanning Tunneling Microscope • Invented in the _______. • _________________an object’s original size. •

Vocabulary 1. Resolution: _______________. Example) unclear pictures= poor resolution 1. Magnification: _______________________________. This is

_____of the Microscope and __________ 4 x 100 x 40 x 10 x __________

__________ -It is very important to note that the eyepiece is a CONVEX lens.

Image Quality When you look at a specimen using a microscope, the quality of

________ Orlando Science Center 2003 March

General Microscope Rules 2) When viewing your specimen, ____________________________. This is always the _________________.

General Microscope Rules 3)_____________________ (bigger knob) (smaller knob) ________________. Once the specimen is in

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LOOKING AT CELLS -History of the Microscope -Types of Microscopes -General Use of the Microscope

Looking at Cells are VERY tiny! ________= ___________(_____) =approximate width of the average fingernail ________=_______________(_____) =equivalent to the width of a pencil tip ________=_________________(_____) = about the length of half of one E. Coli ________= ___________________(______) about the size of a very large molecule ____________________________, which is abbreviated as µm. __________________. Micrometers are also known as_______. Some cells are only half a micron in diameter, which means you could fit two million cells along the width of a meter stick. They are naked to the human eye!

What came first? The cell or the microscope? Anton van Leeuwenhoek’s microscope, established circa 1653 Cells, established billions of years ago

_______________ • A Dutch scientist born in _______. • ------------------------------------------------------. • His new improved microscope was able to see things that no man had ever seen before, i. e. , ___________, ________ and many ______ swimming about in a drop of water. He called these “_________”.

______________ • Robert Hooke, an English scientist, was the first scientist ______________. • When looking at a wine cork under a microscope in ____, he saw something similar to this: Why do you suppose he named these structures “cells”?

1700’s 1800’s 1600’s Today The origin of the microscope is a matter of debate. It is unclear as to who invented the very first microscope.

________Microscope _____________: • The compound microscope has __________________________. • This microscope is ideal for looking at a wide range of______________, though it can only magnify up to ___________ larger. Cells under a compound light microscope.

_____________ An electron microscope is any microscope __________________________ of a specimen. However, they are ___________________________. The specimen is always dead and preserved. There are three types of electron microscopes: 1) _____________Microscope (TEM) – to be discussed… 2) Scanning Electron Microscope SEM) 3) Reflection electron microscope

Transmission Electron Microscope (TEM) • Original electron microscope • Invented in the ____. • ____________________________. • Capable of _______________________. • Ideal for use on cells because TEM’s produce highly magnified 3 -dimensial images of the cell, as we will see in the virtual microscope! http: //school. discoveryeducation. com/lessonplans/interact/vemwindow. html

Scanning Tunneling Microscope • Invented in the _______. • _________________an object’s original size. • ____________________. • _____________ -even cells are too big for the capacity of this amazing instrument!

Vocabulary 1. Resolution: _______________. Example) unclear pictures= poor resolution 1. Magnification: _______________________________. This is done using lenses (like a magnifying glass or eyeglasses). 2. SI units: ______________________ because its eyepiece lens is always ______.

_____of the Microscope and __________ 4 x 100 x 40 x 10 x __________ (3 -4 total) Eyepiece (piece you look through) always has a 10 x lens! Total magnification=_______________ The microscope is currently set on the 10 x objective lens. What is the total magnification?

__________ -It is very important to note that the eyepiece is a CONVEX lens. -This is the same type of lens that is found in our eyes. ______________________.

Image Quality When you look at a specimen using a microscope, the quality of the image you see is assessed by the following: • _______- How light or dark is the image? • _____- Is the image blurry or well-defined? • _______ - How close can two points in the image be before they are no longer seen as two separate points? • _______- What is the difference in lighting between adjacent areas of the specimen?

________ Orlando Science Center 2003 March

General Microscope Rules 2) When viewing your specimen, ____________________________. This is always the _________________. • _______________________when you look through the microscope is called your _________________. • By starting on low power you have the greatest field of vision and it is easier to find your object.

General Microscope Rules 3)_____________________ (bigger knob) (smaller knob) ________________. Once the specimen is in view, ________________________________________.