Logo Diffusion Fundmental experiment Osmosis Fundmental experiment Hematocrit

Logo 실험목록 £ £ £ £ £ Diffusion & Fundmental experiment Osmosis & Fundmental experiment Hematocrit The measurement of Hemoglobin The effect of temperature on metabolism Tracing of glucose in urine Pregnancy test The factors effecting to enzyme kinetics 1, 2 The measurement of protein Anaerobic respiration 2 www. themegallery. com

Logo 보고서 양식 Title £ Name £ Purpose £ Material £ Method £ Result £ Inference £ Reference £ 4 www. themegallery. com

Logo Experiment 1 Diffusion & 인공반투막 5

Logo Result £ Discussion ▶ 확산이란? ▶ Diffusion이 빠른 비이커는? ▶ Diffusion rate에 영향을 주는 factor는? £ 7 www. themegallery. com

Logo £ Experiment Procedure ▶ 시험관에 5% Cu. SO 4 용액을 넣는다. ▶ 쌀알크기의 K 4 Fe(CN)6 결정 첨가. ▶ Cu. Fe(CN)6의 갈색막 관찰 ※ 참고 K 4 Fe(CN)6+Cu. SO 4→Cu. Fe(CN)6+K 4 SO 4 9 www. themegallery. com

Logo Experiment 2 삼투현상 11

Logo 삼투현상 £ Material ▶ 현미경, Slide Glass, Cover Glass, Lancet, 시험관대, 70% Et. OH, 0. 4% Na. Cl, 증류수, 0, 9% Na. Cl, 3% Na. Cl. 12 www. themegallery. com

Logo ※ 참고 0. 4% Na. Cl – Hypotonic sol. (저장액) 0. 85% (0. 9%) – Isotomic sol. (등장액) 3% Na. Cl – Hypertonic sol. (고장액) ※ 용혈 현상? 13 www. themegallery. com

Logo £ Discussion ▶ Isotonic, Hypertonic, Hypotonic sol은 각각 몇 %의 것을 사용했는가? ▶ 각 solution에 있어서 cell size와 cell shape를 비교하라 ▶ 이 실험과 확산, 인공반투막 실험의 총괄적 인 연관성은? 15 www. themegallery. com

Logo Experiment 3 원형질 분리(Plasmolysis & Deplasmolysis) 16

Logo 원형질 분리(Plasmolysis & Deplasmolysis) £ Materials ▶ 면도날 & Needle Na. Cl solution ; 20%, 5%, 2%, Slide glass & Cover glass, Filter paper, Spoid 17 www. themegallery. com

Logo Experiment 4 Hemoglobin(Hb)량 측정 20

Logo £ Material ▶ Sahli-Hemometer(비색관), spoid, 혈액, 피펫 22 www. themegallery. com

Logo Experiment 5 Hematocit 25

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Logo Hematocrit £ Material ▶ 사람의 blood, Centrifuge, Heparinized Capillary Tube £ Procedure ▶ Heparinized Capillary Tube에 혈액을 3/2가량 채움 ▶ Centrifuge/3000 rpm으로 30분간 처리 30 www. themegallery. com

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Logo 온도가 대사에 미치는 영향 Materal Gold fish, 온도계, 수조 £ 40 www. themegallery. com

Logo ▶ 온도별로 측정한 호흡횟수의 data를 grape용지에 grape를 그려라 44 www. themegallery. com

Logo Experiment 7 오줌 속의 당분검출 45

Logo £ Material Benedict시약, test tube, 1%glucos용액, 1% starch용액, 스포이드, 가열 기구, 집게, 시험관, Urine sample. 47 www. themegallery. com

Logo Procedure ▶ Standard 5㎖Benedict를 넣은 3개의 시험관 준비 £ ① 5㎖ of Benedict + 10 drop(Water) ② 5㎖ of Benedict + 10 drop(1%glucose) ③ 5㎖ of Benedict + 10 drop(1%starch) 5분간 중탕 ▶ Urine analysis, 특수 sample, 각자 분단별 sample을 1과 같이 반복 48 www. themegallery. com

Logo Experiment 8 Pregnancy test 49

Logo Pregnancy test £ Introduction ▶ 난자의 형성 & 생식주기 여포(follicle) : 난자를 가지고 있는 조직 Hormone의 영향 ① FSH - Follicle Stimulating Hormone ② Estrogen - 여포의 성숙 ③ LH(황체형성H) - 13~15일 배란될 때 뇌하수체 전엽 ④ Pregesteron - 임신유지 & 태반형성 50 www. themegallery. com

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Logo £ Material HCG AB, Slide Glass, disposable plastic tube, urine sample 53 www. themegallery. com

Logo £ Procedure ▶ Slide glass에 Urine 한방울 ▶ Anti HCG AB을 떨어 뜨린다. ▶ Disosable plastic tube로 섞어준다. ▶ 1분 흔든다. ▶ 시각적으로 응집반응의 여부 관찰 ▶ Positive : 응집 有 임신 ▶ Negative : 응집 無 Agglutination 54 www. themegallery. com

Logo £ Discussion ▶ HCG(Human Chorionic Gonadotropin)? ▶ 임신이란? ▶ 임신에 관여하는 호르몬은? ▶ 항체(Antibody)와 항원(Antigen)이란? 55 www. themegallery. com

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Logo Antigen-antibody Reaction 60 www. themegallery. com

Logo Experiment 9 효소의 작용 61

Logo 효소의 작용 £ Introduction Starch(I 2 KI) Maltose Amylase Maltase Glucose 62 www. themegallery. com

Logo £ Material : 효소(0. 125% amylase), 1% Soluble starch, I 2 KI용액(요오드 용액), Benedict 용액, 0. 5 N HCl(stop solution) 시험관, 시험관대, 피펫, 얼음, 온도계, 항온수조(37℃) 63 www. themegallery. com