LMNA mutated Rabbits A Model of Premature Aging

LMNA -mutated Rabbits: A Model of Premature Aging Syndrome with Muscular Dystrophy and Dilated Cardiomyopathy Sui Tingting 1 ; Liu Di 1 ; Liu Tingjun 1 ; Deng Jichao 1 ; Chen Mao 1 ; Xu Yuanyuan 1 ; Song Yuning 1 ; Ouyang Hongsheng 1 ; Lai Liangxue 1, 2 ; Li Zhanjun 1 ; 1 Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun 130062, China ; 2 Key Laboratory of Regenerative Biology, and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China ; Figure 6. Lipodystrophy of LMNA -KO rabbits. A Dysplasia of the adipose tissue in LMNA -KO rabbits by anatomy analysis. B The liver of LMNA -KO rabbits and WT controls. C H#cod#x 00026; E-staining and Oil red staining of the brown adipose tissue from LMNA -KO rabbits and WT rabbits. D H#cod#x 00026; E-staining of liver sections from WT and LMNA -KO rabbits. E Gene expression of PPAR#cod#x 00261; , SREBF 1 , GLUT 4 , FABP 4 , and ADIPOQ was determined by q. RT-PCR. WT, WT control; LMNA KO, LMNA gene knockout rabbit. All experiments were repeated for three times for each gene. Data are presented as the mean #cod#x 000 B 1; SEM and analyzed by t-tests using Graphpad Prism software 6. 0. #cod#x 0002 A; p #cod#x 0003 C; 0. 05; #cod#x 0002 A; p #cod#x 0003 C; 0. 01; #cod#x 0002 A; p null, 0(0), null-null. Doi: 10. 14336/AD. 2018. 0209 #cod#x 0003 C; 0. 001. Scale bar, 50 #cod#x 003 BC; m.