Lesson 7 2 Bacteriology Specimen Collection and Processing

Lesson 7 -2 Bacteriology Specimen Collection and Processing

Overview Importance of collection and transport methods Types of cultures commonly performed Throat Nasal Urine Wound Respiratory Stool Blood

Specimen Collection Reasons for ordering cultures See Table 7 -10 Specimen collection manual Specimen collection method See Table 7 -11

Collection and Transport Supplies Support a variety of specimens Support anaerobic and aerobic organisms Protect viability during transport Approved leak-proof containers See Table 7 -12

Collection and Transport Supplies

Specimen Collection Safety precautions Standard Precautions Disinfect setup area Quality assessment Follow procedure manual Label appropriately See Table 7 -13

Specimen Collection Throat culture Swab tonsils only Inoculate plate or use transport media Nasal culture MRSA carriers Bordetella pertussis Other

Specimen Collection

Specimen Collection Urine culture Midstream, clean-catch Transport system

Specimen Collection Wound culture Aspirate preferred Immediately inoculate transport medium Culture for anaerobes and aerobes

Specimen Collection Respiratory culture – sputum Rinse mouth before collection Klebsiella pneumoniae Streptococcus pneumoniae

Specimen Collection Stool culture Specimen must not be uncontaminated Special media to support Salmonella, Shigella, E. coli Vibrio Campylobacter Clostridium difficile

Specimen Collection Blood culture Phlebotomist wears sterile gloves Cleanse site with alcohol then iodine Various styles of collection bottles