Lesson 1 10 The Microscope Light Microscopes Image

Lesson 1 -10 The Microscope

Light Microscopes Image illuminated by light source • Bright-field • Phase contrast • Epi-fluorescence

Light Microscopes Image illuminated by light source • • Dark field DIC Polarizing Confocal laser scanning

Electron Microscopes Electron beam produces image

Electron Microscopes Transmission (TEM) • Electron beam passes through image Scanning (SEM) • Electrons bounce off specimen surface

Clinical Microscope Indispensable

Parts of the Microscope Oculars • Reticles Objective lenses • Numerical aperture • Plan and achromatic lenses • Magnification

Image Magnification Lens Magnification × Ocular Mag. = Total Mag. Low 10× 100× High 40× 10× 400× Oil 100× 1000×

Parts of the Microscope Light, condenser, iris diaphragm Field diaphragm Coarse and fine adjustments Stage

How the Image Is Produced Magnified Focused Directed into oculars

Using the Microscope Safety Quality assessment • Care and cleaning of lenses • Immersion oil

Using the Microscope Adjusting oculars • Dioptic • Interpupillary Alignment of illumination

Using the Microscope Focusing • Low power objective • High power objective • Oil immersion objective Use only with immersion oil Transporting and Storing

Using the Microscope