Learning how to use the microscope There are

Learning how to use the microscope

There are 2 kinds of microscopes: Simple: one lens (magnifying glass) Compound: 2 or more lenses *We use compound microscopes in class!

Parts of the Microscope 1. Revolving Nosepiece

Parts of the Microscope 2. Lens/Objective

Parts of the Microscope 3. Stage

Parts of the Microscope 4. Disc Diaphragm (controls amount of light) 1 = lowest setting/ 5 = highest setting

Parts of the Microscope 5. Light source

Parts of the Microscope 6. Base

Parts of the Microscope 7. Eyepiece

Parts of the Microscope 8. Arm

Parts of the Microscope 9. Stage Clips

Parts of the Microscope 10. Coarse Focus/ Adjustment (Big Knob)

Parts of the Microscope 11. Fine Focus/ Adjustment (Small Knob)

Calculating Magnification Multiply lens powers together!

• Eyepiece: Eyepiece 10 X by itself • Low Power: 4 X; red color; and a TOTAL magnification of 40 X mainly used for large objects/scanning • Medium Power: 10 X, yellow color; TOTAL magnification of 100 X • High Power: 40 X; blue color; and a TOTAL magnification of 400 X; mainly used for smaller objects/details

When you are ready to start: üMake sure it is on LOW power! üThe stage should be all the way down (you focus by slowly moving it up!) üCheck the light setting (should be at 1 -2 to start)

Other Things to Consider: Carrying the Microscope: Always use 2 hands!

Other Things to Consider: Never use the COARSE FOCUS KNOB while on HIGH power.

When putting the microscope away (at the end of the day): üIt should be on LOW power. üThe cord should be WRAPPED around the base. üThe stage should be all the way DOWN (do this with the coarse focus knob!) üThe light switch should be OFF

TROUBLESHOOTING What if you can’t get your object into focus? • Check that you have one of the lenses clicked into place • Check that your disc diaphragm is in place (not IN BETWEEN settings)

TROUBLESHOOTING What if you can’t get your object into focus? • Clean off your lenses using LENS PAPER. (Also, dirty slides are hard to focus on; so clean them, too!) • GO BACK TO LOW POWER AND START OVER!

• Instead of a beam of light, these microscopes use a beam of electrons as their source of illumination! Instead of using glass lenses to focus the light, they use a MAGNETIC field to focus the beam of electrons! • Used to see things at much higher level of detail • Can magnify images up to 1, 000 times!

There are 2 kinds: • SCANNING ELECTRON MICROSCOPES (SEM): Allows you to see the SURFACES of objects

There are 2 kinds: • TRANSMISSION ELECTRON MICROSCOPES (TEM): Allow you to see THROUGH an object.

Visit this Website to check out some really cool Electron Microscope Images and then answer the questions on your paper! http: //www. pbrc. hawaii. edu/bemf/microangela/