Leafy Vegetables 2 nd annual report 26 27

Leafy Vegetables 2 nd annual report: 26 -27 Nov 2008, Sweden, Nordgen WP 3: Evaluation Cadot Valérie Coquin Pascal GEVES : Variety and seed, study and control group Groupe d’Étude et de contrôle des Variétés Et des Semences Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

A. Objectives u Evaluation for pests and diseases resistance u Evaluation for quality and abiotic characters u Inclusion in the databases to enable a more sustainable production methods and Leafy a better product Vegetables, quality WP 3, Leafy 14 th Vegetables, February WP 3

Deliverables Deliverable date WP 3 for WP 3 Deliverable description Status Partner responsible Done except for P 12 P 0, P 1, P 2, P 3, P 4, P 5, P 7, P 9, P 10, P 12, P 13 Not yet due P 1, P 4, P 7, P 9, P 10, P 13 Year 1 D 16; month 12 Seeds of the selected material of the four crop groups sent to the partners involved Year 4 D 17; month 45 115 accessions of lettuce evaluated D 18; month 45 320 accessions of spinach evaluated Not yet due P 0, P 5 D 19; month 45 265 accessions of chicory evaluated Not yet due P 2, P 12 D 20; month 45 49 accessions of minor crops evaluated Not yet due P 3, P 5, P 12 Not yet due P 0, P 1, P 3, P 5, P 9, Evaluation data entered in a fixed D 21; month 45 format to be sent to the database Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3 managers P 2, P 4, P 7, P 10,

Milestones for WP 3 Milestone date Milestone description Status Partner responsible Done except for P 12 P 0, P 1, P 2, P 3, P 4, P 5, P 7, P 9, P 10, P 12, P 13 On going P 0, P 1, P 2, P 4, P 5, P 7, P 9, P 10, P 12, P 13 P 1, P 4, P 7, P 9, P 10, P 13 Year 1 month 12 Material selected for the evaluation activities of the four crop groups (see WP 1) Year 2 month 18 Evaluations started Year 3 month 36 70 accessions of lettuce evaluated Not yet due month 36 250 accessions of spinach evaluated Not yet due P 0, P 5 month 36 170 accessions of chicory evaluated Not yet due P 2, P 12 30 accessions of minor crops Not yet P 3, P 5, Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

Milestones in 2010 of evaluation for pests and diseases resistance u Lettuce: 60 accessions – Bremia lactucae : u P 4 (CZ): 30 accessions with 20 isolates by seedling tests, u P 10 (GBR): 17 accessions for field resistance – Root-knot nematodes (Meloidogyne sp. ): P 13 (SLO): 10 -20 u Spinach: 250 accessions (3 -5 races) – Downy mildew (Peronospora farinosa) P 0 (NLD) : 250 u Chicory: 165 accessions – Alternaria cichorii P 2 (FRA): 60 – Sclerotinia sclerotiorum P 2 (FRA): 30 – Thielaviopsis basicola P 2 (FRA): 75 Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

Milestones in 2010 of evaluation for quality and abiotic characters u Lettuce: 45? (or less) instead of 55 accessions? – Post harvest discoloration – Winter hardiness 15 – Taste up? u P 1(GBR): 24 P 7 (AUT): 6 instead of P 7 (AUT): 6? / P 9: 10 given Spinach: 70 accessions – Nitrate, oxalic acid, dry matter u Chicory: 100 accessions – Sesquiterpenes u P 5 (DAN, SW) P 12 (ITA): 100 Rucola: 49 accessions – Nitrate, matter P 5 (DAN, SW): 20 Leafydry Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3 – Glucosinolate content P 12 (ITA): 20 to 40

Evaluation in 2008 for P 0 (CGN) u What has been done: – – Evaluation of 263 spinach accessions for 3 Peronospora farinosa pathotypes (Pf 5 and Pf 7 and ? ) > 250 accessions planned. Scale of notation: u Absolute resistance on cotyledons (1: 100% resistant ; 9: 100% susceptible) u Partial resistance on first leaves(1: 100% resistant ; 9: 100% susceptible) u What will be done: Send the results of Pf 5 and Pf 7 to WP 3 leader at the end of 2008. Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

Evaluation in 2008 for P 1: (WHRI) u u What has been done: In 2007: evaluation of 28 lettuce accessions for 2 types of discoloration : browning and pinking (> 24 in the proposal) The analysis of the data has been carried out. What will be done: Data to be provided to WP 3 coord. & lettuce data base manager and WP 1 coordinator Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

u Évaluation in 2008 for P 2 (GEVES) What has been done: - Alternaria cichorii (GEVES Brion): Evaluation of 22 chicory accessions - Sclerotinia sclerotiorum (by FNPE): Evaluation on going of 14 chicory accessions in february 2009 during the forcing period. Alternaria cichorii - Thielaviopsis basicola (by SNES): Evaluation on going of 17 chicory accessions. In July 2008 : 1 st evaluation giving discriminating results but low degree of attack ; so 2 nd test in 2009 u What will be done: Provide the data to the WP 1 coordinator Go on the evaluations in 2009 and 2010 : - Alternaria cichorii: 20 accessions in 2009 and 18 in 2010 ( total of 60) - Sclerotinia sclerotiorum: 16 Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3 accessions/30 Sclerotinia sclerotiorum Thielaviopsis basicola:

Evaluation in 2008 for P 4 (Palacky) u u What has been done: In 2008, evaluation of 30 lettuce accessions for response to 3 Bremia lactuca isolates What will be done: Evaluation of these 30 lettuce accessions for response to 17 other isolates of Bremia lactucae? How many strains in 2009 and in 2010? Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

Evaluation in 2008 for P 5 (Nordgen) u u What has been done: - Rucola (Eruca) 30 accessions collected and submitted to chemical evaluations (nitrate, oxalic acid) ; results not yet available. - Spinach : 55 accessions collected and submitted to chemical evaluations ; results not yet available. Bottlenecks: Not sure to test the 5 accessions of Atriplex (orache) Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

Evaluationin 2008 for P 7 (Arche Noah) u What has been done: Evaluation on going for winter hardiness: – 6 lettuce accessions planted in october. – Criteria for evaluating winter hardiness has been developed. u What will be done: 6 accessions for taste? Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

Evaluation for P 10 (HDRA) u What has been done: Evaluation in the field of 16 lettuce accessions (instead of 17) for Bremia lactucae – – u 1 variety came back to the National List Very high disease pressure; a couple of accessions having almost no downy mildew and having good result in the taste test What will be done: in 2009: no accessions of lettuce tested Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

Evaluation in 2008 for P 12 (University of Bologna) u What has been done: – Evaluation of 37 Rucola accessions (Eruca sativa, Diplotaxis tenuifolia) for glucosinolate content. – Templates sent to WP 3 coordinator ; discussion about Instcode (Sativa? ) u What will be done: – to evaluate chicory for sesquiterpenes in 2009 and 2010: 100 accessions, discussion about the choice of accessions Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

Evaluation for P 12 Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

Evaluation in 2008 for P 13 (KIS) u What has been done: – Evaluation of 7 accessions for tolerance-resistance to root-knot nematodes (Meloïdogyne ethiopica). – None resistance to root –knot nematode but 3 accessions suggesting tolerance u What will be done: – Transmission of data to WP 3 coordinator and database manager Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

WP 3 : what has been done in 2008 Pests & diseases resistance evaluation For P 4: 15% isolates evaluated Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

WP 3 : what has been done in 2008 Quality & abiotic characters evaluation 136% (67/49) 75% (34/45) 78% Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3

WP 3 : what has been done in 2008 Pests & diseases resistance + Quality & abiotic characters evaluation 136% (67/49) 99% (318/320) 83% (87/105) 7/14 16/17 6/6 30 acc. 3/20 str. 37 55/70 263/250 20% (53/265) 28/24 Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3 30

WP 3 : what will be done in 2009 Pests & diseases resistance + Quality & abiotic characters evaluation P 4: 30 acc. ? str. of B. l. ? Leafy Vegetables, WP 3, Leafy 14 th Vegetables, February WP 3 105 inst. of 115