Laser Scan Confocal MicroscopeLSCM Leica Flow cytometryBD Transfection

形态暨影像公共平台 • 激光共聚焦显微镜 (Laser Scan Confocal Microscope，LSCM， Leica) • 流式细胞仪 (Flow cytometry，BD)

Transfection • Incubate the cells: 30 -40% or 70%-80% confluence. • Transfection: lipofectamine 2000 • Transient transfection: used for imaging directly. • Select stable cell lines: 48 h, G 418, 2 -3 weeks.

Check whether p. Ds. Red 1 -mito can probe mitochondria in CMs by using 生理楼 2012. 09. 04 confocal microscope p. Ds. Red 1 Mito Green Merge X 63

2. 5 h