Laboratory Safety Rules While working in the science

Laboratory Safety Rules

While working in the science laboratory, you will have certain important responsibilities that do not apply to _______ other classrooms. You will be working with materials and apparatus which, if handled carelessly or improperly, have injury or the potential to cause ______ discomfort to someone else as well as yourself.

A science laboratory can be a safe place in which to work if you, the student, are foresighted, alert, and cautious. Violating any of the following regulations will result in you being suspended from class or permanently removed from the class. The following practices will be followed:

instructor must be present during the 1. An _____ performance of all laboratory work. accident to the teacher 2. Report any _____ minor immediately, no matter how_______, including reporting any burn, scratch, cut, or corrosive liquid on skin or clothing. 3. Prepare for each laboratory activity by reading all instructions before coming to _______ directions implicitly and class. Follow all _____ modification intelligently. Make note of any _____ in procedure given by the instructor.

4. Any science project or individually planned experiment must be approved by the teacher. _____ 5. Use only those materials and equipment authorized by the instructor. _____ immediately of any 6. Inform the teacher______ equipment not working properly. spill on 7. Clean up any nonhazardous _____ immediately the floor or workspace _____.

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eye protection as 8. Wear appropriate _______, directed by the instructor, whenever you are working in the laboratory. Safety goggles must be worn during hazardous activities involving caustic/corrosive _____ chemicals, heating of liquids, and other activities that may injure the eyes.

9. Splashes and fumes from hazardous chemicals present a special danger to contact lenses Therefore, wearers of _______. students should preferably wear regular glasses (inside splash -proof goggles, when appropriate) during all class activities or purchase personal splashproof goggles and wear them whenever exposure to chemicals or chemical fumes is possible. Students with open skin wounds on hands must wear gloves or be excused from the laboratory activity.

carry hot equipment or 10. Never ______ group dangerous chemicals through a ______ of students. labels and equipment 11. Check ______ instructions carefully. Be sure correct used in the proper manner. items are ______ chemicals being used 12. Be aware if the _____ are hazardous. Know where the material MSDS is and what it safety data sheet (______) indicates for each of the hazardous chemicals you are using.

taste anything or touch 13. Never ______ chemicals with the hands, unless specifically instructed to do so. _____ 14. Test for odor of chemicals only by waving your hand above the container ______ distance and sniffing cautiously from a ____. in the laboratory or 15. Eating or drinking ___ NOT from laboratory equipment is ____ permitted. mechanical pipette filler (never 16. Use a _____ the mouth) when measuring or transferring small quantities of liquid with a pipette.

17. When heating material in a test tube, look into the tube or point it in do not _____ the direction of any person during the process. reagents back into bottles, 18. Never pour ____ exchange stoppers of bottles, or lay stoppers on the table. acids always pour 19. When diluting _____, water never the reverse. acids into ______, Combine the liquids slowly while stirring to distribute heat buildup throughout the mixture.

20. Keep _______ hands away from face, eyes, and clothes while using solutions, specimens, equipment, or materials in the laboratory. Wash hands as necessary conclusion and wash thoroughly at the _____ of the laboratory period. 21. To treat a burn from an acid or alkali, immediately with wash the affected area ______ plenty of running water. If the eye is involved, irrigate it at the eyewash station without interruption for 15 minutes. Report the incident to your instructor immediately.

location of the emergency 22. Know the ____ shower and eyewash station, fire blanket, fire extinguisher, fire alarm box, and exits. 23. Know the proper fire and earthquake procedures drill ______. wrist 24. Roll long sleeves above the _____. Long, hanging necklaces, bulky jewelry, and excessive and bulky clothing should not be worn in the laboratory 25. Confine long hair during a _____ activity.

cover the toes, rather 26. Wear shoes that _____ than sandals, in the laboratory. clean Floors and 27. Keep work areas _____. aisles should be kept clear of equipment and materials. burners only as instructed 28. Light gas ____ volatile by the teacher. Be sure no _______ materials (such as alcohol or acetone) are being used nearby. Use a burner with extreme caution. Keep your head and ____ clothing away from the flame and turn it off when not in use.

29. Use a fire blanket (stop, drop, and roll) extinguish any flame on a person. to _____ 30. Dispose of laboratory waste as instructed by the teacher. Use separate, _____ designated containers (not the wastebasket for the following: ______) §Matches, litmus paper, wooden toothpicks and so on splints, _____, Broken and waste glass §_______ §Rags, paper towels, or other absorbent materials used in the cleanup of flammable solids or liquids _____ liquids and solids §Hazardous/toxic ______

31. Place books, purses, and such items in the designated storage area. Take only laboratory manuals and notebooks into _____ working area. the _______ 32. Students are not permitted in storage rooms or teachers' laboratory _______ workrooms without the approval of the teacher. ____ 33. To cut small diameter glass tubing, use file or tubing cutter to make a deep a ____ scratch. Wrap the tubing in a paper towel before breaking the glass away from you with your thumbs. Fire polish all ends.

visual 34. Hot and cold glass has the same _____ appearance. Determine whether an object is hot by bringing the back of your hand close to the object. 35. Match hole sizes and tubing when inserting glass tubing into a stopper. If necessary, expand the hole first by using an appropriate size cork borer. Lubricate the stopper hole and glass tubing with water or glycerin to ease insertion, using towels to protect the hand. Carefully twist ______ (never push) glass tubing into stopper holes.

broken glass from the 36. Remove all ____ work area or floor as soon as possible. Never handle broken glass with bare hands; use a counter brush _____ and dustpan. 37. Report broken glassware, including thermometers, to the instructor immediately. _____. 38. Operate electrical equipment only in a dry hands. dry area and with _____ 39. When removing an electrical plug from plug not the its socket, pull the ______, electrical cord.

40. Always approach laboratory serious and courteous experiences in a _______ manner. clean the laboratory area 41. Always ______ before leaving. 42. Students and teacher wash hands with soap and water before leaving the ______ laboratory area.

43. When heating volatile or flammable materials, use a water bath; that is, heat the materials in or over heated water, using a hot plate to heat the water. Extinguish all open flames. _____ caution in using scissors, 44. Exercise _______ scalpels, dissecting needles, and other sharp-edged instruments. Pass them with handles extended when handing them to other persons.

immediately of any 45. Inform the teacher ______ equipment not working properly. 46. Use the fume hood whenever noxious, fumes are produced corrosive, or toxic _______ or released. clean before 47. Be sure all glassware is ______ use. Clean glassware thoroughly ______ after use. Residue may cause errors in new violent reaction experiments or cause a _______ or explosion.

The following actions will result in being dropped from the class: Misuse of safety equipment including, but _______ not limited to – eyewash stations – emergency showers – fire extinguishers – fire blanket Vandalizing school equipment or furniture. _____ Endangering or causing harm to others. _____

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LABORATORY SAFETY • Chemical Hazard Label • MSDS • Safety Quiz Courtesy Christy Johannesson www. nisd. net/communicationsarts/pages/chem

Safety Symbols

NFPA CHEMICAL HAZARD LABEL FLAMMABILITY HEALTH REACTIVITY (STABILITY) SPECIAL

NFPA CHEMICAL HAZARD LABEL 2 4 0 4 3 Least Serious 4 Flammable vapor which burns readily 0 Substance is stable Most Serious

NFPA CHEMICAL HAZARD LABEL Burns readily. Diborane Severe health risk. 4 4 3 W Avoid water. May detonate with heat or ignition.

NFPA Label Chem. name Fire Hazard (red) Flash Point Temp. 4 – below 73 o. F Temp. 3 – 73 to 100 o. F – v. flam. 2 – 101 to 200 o. F – flam. 1 – over 200 o. F - slightly combustible 0 – will not burn Health (blue) 4 – deadly 3 – extreme danger 2 – hazardous 1 – slightly hazardous 0 – normal material Reactivity (yellow) Specific Hazard OXY – oxidizer ACID – acid ALK – alkali COR – corrosive W – use no water RAD – radiation hazard 4 – may detonate 3 – shock or heat may detonate 2 – violent chem. reaction 1 – unstable if heated 0 – stable

NFPA CHEMICAL HAZARD LABEL Complete Label for Acetone

NFPA CHEMICAL HAZARD LABEL Complete Label for Phosphine

MSDS • Material Safety Data Sheet • On file for all purchased chemicals. • Includes all information shown on a chemical label and more. • Different formats are used by different chemical companies.

MSDS

Health Hazard 4 Very short exposure could cause death or serious residual injury even though prompt medical attention was given. 3 Short exposure could cause serious temporary or residual injury even though prompt medical attention was given. 2 Intense or continued exposure could cause temporary incapacitation or possible residual injury unless prompt medical attention is given. 1 Exposure could cause irritation but only minor residual injury even if no treatment is given. 0 Exposure under fire conditions would offer no hazard beyond that of ordinary combustible materials.

FLAMMABILITY 4 Will rapidly or completely vaporize at normal pressure and temperature, or is readily dispersed in air and will burn readily. 3 Liquids and solids that can be ignited under almost all ambient conditions. 2 Must be moderately heated or exposed to relatively high temperature before ignition can occur. 1 Must be preheated before ignition can occur. 0 Materials that will not burn.

1 Prior to 1996, this section was titled "Reactivity". The name was changed because many people did not understand the distinction between a "reactive hazard" and the "chemical reactivity" of the material. The numeric ratings and their meanings remain unchanged. INSTABILITY 1 4 Readily capable of detonation or of explosive decomposition or reaction at normal temperatures and pressures. 3 Capable of detonation or explosive reaction, but requires a strong initiating source or must be heated under confinement before initiation, or reacts explosively with water. 2 Normally unstable and readily undergo violent decomposition but do not detonate. Also: may react violently with water or may form potentially explosive mixtures with water. 1 Normally stable, but can become unstable at elevated temperatures and pressures or may react with water with some release of energy, but not violently. 0 Normally stable, even under fire exposure conditions, and are not reactive with water.

OX This denotes an oxidizer, a chemical which can greatly increase the rate of combustion/fire. Unusual reactivity with water. This indicates a potential hazard using water to fight a fire involving this material. This section is used to denote special hazards. There are only two NFPA 704 approved symbols

ACID This indicates that the material is an acid, a corrosive material that has a p. H lower than 7. 0 ALK This denotes an alkaline material, also called a base. These caustic materials have a p. H greater than 7. 0 This denotes a material that is corrosive (it could be either an acid or a base). The skull and crossbones is used to denote a poison or highly toxic material. See also: CHIP Danger symbols. The international symbol for radioactivity is used to denote radioactive hazards; radioactive materials are extremely hazardous when inhaled. Indicates an explosive material. This symbol is somewhat redundant because explosives are easily recognized by their Instability Rating.