Laboratory of Mol Biology and Immunology Laboratory of
Laboratory of Mol. Biology and Immunology
Laboratory of Molecular Biology and Immunology Research Interests Konstantinos Poulas’ Group 1. Cloning, expression, purification and biochemical characterization of recombinant fragments – “Protein Repair” 2. Toxicological and preclinical studies on non-combustible products: e-cigs and Hn. B products 3. Structural studies of proteins by X-ray crystallography and molecular modelling. Innovative crystallization methods. 4. Databases for autoimmune and genetic diseases. 5. Innovative technology NCCT (Non-contact Current Transfer) for wound/burns healing - Athletic recovery. Studies at cellular and molecular level. 6. Food Innovation
Laboratory of Molecular Biology and Immunology Research Interests Konstantinos Poulas’ Group 1. Toxicological and preclinical studies on non-combustible products: e-cigs and Hn. B products
Routes of administration are inhalation (smoking or vaporizing) or orally by consumption of oil extracts. Oil products can reliably be manufactured to produce a medical product with high reproducibility and repeatability, with consistent amounts of active ingredients. Cannabis flowers cannot offer equivalence accuracy and consistency. Development of herbal medicines encompasses fundamental challenges that require specialized research. The plant is composed of diverse components and hence advanced analytical tools are required in order to differentiate them.
Research Subjects on e-cig/HNB • Toxicology - Metals Farsalinos KE, Voudris V, Poulas K. Are metals emitted from electronic cigarettes a reason for health concern? A risk-assessment analysis of currently available literature. Int J Environ Res Public Health. 2015 May 15; 12(5): 5215 -32. Kamilari E, Farsalinos K, Kontoyiannis C, Poulas K, Orkoula M. Detection and quantitative determination of heavy metals in e-cig refill liquids using total reflection X-ray fluorescence spectrometry. Food and Chemical Toxicology. In press • Toxicology – Aldehydes/nitrosamines Farsalinos KE, Voudris V, Spyrou A, Poulas K. E-cigarettes emit very high formaldehyde levels only in conditions that are aversive to users: A replication study under verified realistic use conditions. Food Chem Toxicol. 109(Pt 1): 90 -94. Farsalinos KE, Voudris V, Poulas K. E-cigarettes generate high levels of aldehydes only in 'dry puff' conditions. Addiction. 2015 Aug; 110(8): 1352 -6 Farsalinos KE, Gillman G, Poulas K, Voudris V. Tobacco-Specific Nitrosamines in Electronic Cigarettes: Comparison between Liquid and Aerosol Levels. Int J Environ Res Public Health. 2015 Jul 31; 12(8): 9046 -53. Farsalinos KE, Gillman IG, ……Brown SE, Poulas K, Voudris V. Nicotine levels and presence of selected tobacco-derived toxins in tobacco flavoured electronic cigarette refill liquids. Int J Environ Res Public Health. 2015 Mar 24; 12(4): 3439 -52.
Research Subjects on e-cig/HNB • Biochemistry - Nicotine Farsalinos KE, Gillman G, Poulas K, Voudris V. Changes in puffing topography and nicotine consumption depending on the power setting of electronic cigarettes. Int J Environ Res Public Health. In press Farsalinos KE, Yannovits N, …. . , Poulas K. Nicotine delivery to the aerosol of a heat-not-burn tobacco product: comparison with a tobacco cigarette and e-cigarettes. Nicotine Tob Res. 2017 Jun 16. doi: 10. 1093/ntr/ntx 138. Farsalinos KE, Yannovits N, ……, Poulas K. Protocol proposal for, and evaluation of, consistency in nicotine delivery from the liquid to the aerosol of ecigarettes atomizers: regulatory implications. Addiction. 2016 Jun; 111(6): 1069 -76. Farsalinos KE, . . ……. S, Poulas K, Voudris V. Nicotine absorption from electronic cigarette use: comparison between experienced consumers (vapers) and naïve users (smokers). Sci Rep. 2015 Jun 17; 5: 11269. doi: 10. 1038/srep 11269. Farsalinos KE, Gillman IG, ……Poulas K, Voudris V. Nicotine levels and presence of selected tobacco-derived toxins in tobacco flavoured electronic cigarette refill liquids. Int J Environ Res Public Health. 2015 Mar 24; 12(4): 3439 -52. • Epidemiology/regulations Yfantis D, Lagoumintzis G, Farsalinos K, Poulas K. Analysis and Development of Health Policies using Modern Informatics Tools: Products that Reduce the Health Risks of Tobacco Use. In press Farsalinos KE, Poulas K, Voudris V, Le Houezec J. Prevalence and correlates of current daily use of electronic cigarettes in the European Union: analysis of the 2014 Eurobarometer survey. Intern Emerg Med. 2017 Mar 4. doi: 10. 1007/s 11739 -017 -1643 -7. Farsalinos KE, Poulas K, Voudris V, Le Houezec J. E-cigarette use in the European Union: millions of smokers claim e-cigarettes helped them quit. Addiction. 2017 Mar; 112(3): 545 -546. Farsalinos KE, Poulas K, Voudris V, Le Houezec J. Electronic cigarette use in the European Union: analysis of a representative sample of 27 460 Europeans from 28 countries. Addiction. 2016 Nov; 111(11): 2032 -2040.
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