Lab Exercise 17 Biochemical Differentiation of some Medically

Lab Exercise 17: Biochemical Differentiation of some Medically Important Gram-negative Bacilli

Mac. Conkey Agar Left: Shigella - result Right: Escherichia + result

Carbohydrate Fermentation Tests Glucose w/ Durham Fermentation Tube

Carbohydrate Fermentation Tests Sucrose Fermentation

Triple Sugar Iron -/+/+

IMVi. C Series Indole Test broth with tryptophan

IMVi. C Series Methyl Red Test

IMVi. C Series Voges-Proskauer Test Leave uncapped for 15 -20 minutes……. . MRVP broth

IMVi. C Series Citrate Utilization Test - +

Urease Test Negative result (acidic) Uninoculated urea agar Positive result (alkaline) Slow positive

Motility Test

Mac. Conkey agar 1. Which plate could have E. coli on it? 2. Which plate shows the presence of a lactose-fermenter? 3. Which plate could have Salmonella on it? A 4. Which plate shows the presence of a lactose non-fermenter? B 4. B 2. A 3. B 1. A Answers:

What is the name of the lab procedure demonstrated here? Answer: Stabbing the butt!

Use the chart on p. 181 and or the flow chart on p. 182 of your lab manual to figure out who I am! M V C Answer: Shigella sonnei I U

Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! U M V C Answer: Escherichia coli I

Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! U M V C Answer: Serratia marcesens I

Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! M V C Answer: Klebsiella pneumoniae I

Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! M V C Answer: Salmonella typhimurium I U

Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! M V C Answer: Proteus vulgaris I U

Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am! U M V C Answer: Enterobacter aerogenes I