Lab 7 Fasciolopsis buski Clonorchis sinensis Heterophyes heterophyes

Lab. 7: Fasciolopsis buski Clonorchis sinensis Heterophyes heterophyes paragonimus westermani

Fasciolopsis buski

Fasciolopsis buski A. Adult similar, but larger than F. hepatica.

Intestinal fluke Fasciolopsis buski (Fasciolopsiasis). • Large fleshy worm Size 20 -75 mm X 8 -20 mm. • Oral sucker is smaller than the ventral sucker. • Testes are highly branched in the posterior 3/5 of the body. • Small branched ovary. • Short convoluted uterus.

Fasciolopsis buski Oral sucker Intestinal ceca

Adult fluke of Fasciolopsis buski The adult flukes range in size: 20 to 75 mm by 8 to 20 mm

Clonorchis sinensis

Clonorchis sinensis A. Adult: Ø Long, lanceolate body Ø Digestive tract: • simple, • esophagus short, • 2 unbranched intestinal ceca Ø Testes 2, branched. Ø Vitelline gland, many in lateral position. Ø Uterus coiled. Ø Ovary single.

Oral sucker Intestinal ceca Ventral sucker Vitelline gland Uterus Ovary 2 branched testes

Adults of Clonorchis sinensis

Clonorchis sinensis B. Egg ØDistinct operculum. ØSmall knob at the opposite end. ØContains a fully developed miracidium when discharged in the faeces.

Iodine s. Saline s. Clonorchis sinensis : Eggs

Heterophyes heterophyes

Heterophyes heterophyes A. Adult Ø Size very small. Ø Intestinal ceca unbranched. Ø Suckers 3, oral, ventral and genital. Ø Genital sucker adjacent to ventral sucker. Ø Testes 2 rounded unbranched. 2 rounded testes

Oral sucker Intestinal ceca Acetabulum Genital sucker Ovary 2 rounded testes

Pulmonary fluke paragonimus westermani (Paragonimiasis)

paragonimus westermani A. Adult: v Shape rounded 7. 5 -12 mm long X 4 -6 mm wide & 3. 5 -5 mm deep. v wave ceca. v Oral sucker & acetabulum almost equal in size. v Testes slightly branched parallel to each other. v Ovary single, anterior to testes.

Uterus Ovary 2 testes