Lab 6 Trematodes Fasciola hepatica Adult worm in

Lab. 6: Trematodes Fasciola hepatica

Adult worm in human & other vertiberates (sexual reproduction) Schistosoma Metacercaria (other trematodes) Cercaria free in fresh water 1 st & 2 nd generation of Redia (snail) Eggs in fresh water (3 -7 weeks) Miracidium (swimming free in water ) Sporocyst (soft tissue of the aproprate intermediat host"snail“ (asexual reproduction)

Fasciola hepatica

Fasciola hepatica A. Adult: v large and broadlyflattened, v measuring up to 30 mm long and 15 mm wide. v. The anterior end is cone-shaped. v. Extensive branching of: intestinal ceca, testes and vitelline follicles.

Fasciola hepatica A: Adult Carmine s. Fasciola hepatica Adult 20 – 30 mm L. X 8 – 13 mm B, ( grey – brown )

Fasciola hepatica B-Ovum Large , Single celled Unembryonated with operculum

Fasciola hepatica C-Miracidium Ciliated, Swimming in water Many celled.

Fasciola hepatica D-Redia Sac - like structure with germ cells, Clusters of germ cells differentiate into daughter redia or cercaria

Fasciola hepatica E. Cercaria: Anterior trunk Posterior tail, Leaving snail to swim in water.

Fasciola hepatica F. Metacercaria: Globose structures Encysted on aquatic vegetation.

Miracidium sporocysts Egg Rediae Cercaria Metacercaria stages from Fasciola hepatica life cycle

Lab. 7: Fasciolopsis buski Clonorchis sinensis Heterophyes heterophyes paragonimus westermani

Fasciolopsis buski

Fasciolopsis buski A. Adult similar, but larger than F. hepatica.

Intestinal fluke Fasciolopsis buski (Fasciolopsiasis). • Large fleshy worm Size 20 -75 mm X 8 -20 mm. • Oral sucker is smaller than the ventral sucker. • Testes are highly branched in the posterior 3/5 of the body. • Small branched ovary. • Short convoluted uterus.

Fasciolopsis buski Oral sucker Intestinal ceca

Adult fluke of Fasciolopsis buski The adult flukes range in size: 20 to 75 mm by 8 to 20 mm

Clonorchis sinensis

Clonorchis sinensis A. Adult: Ø Long, lanceolate body Ø Digestive tract: • simple, • esophagus short, • 2 unbranched intestinal ceca Ø Testes 2, branched. Ø Vitelline gland, many in lateral position. Ø Uterus coiled. Ø Ovary single.

Oral sucker Intestinal ceca Ventral sucker Vitelline gland Uterus Ovary 2 branched testes

Adults of Clonorchis sinensis

Clonorchis sinensis B. Egg ØDistinct operculum. ØSmall knob at the opposite end. ØContains a fully developed miracidium when discharged in the faeces.

Iodine s. Saline s. Clonorchis sinensis : Eggs

Heterophyes heterophyes

Heterophyes heterophyes A. Adult Ø Size very small. Ø Intestinal ceca unbranched. Ø Suckers 3, oral, ventral and genital. Ø Genital sucker adjacent to ventral sucker. Ø Testes 2 rounded unbranched. 2 rounded testes

Oral sucker Intestinal ceca Acetabulum Genital sucker Ovary 2 rounded testes

Pulmonary fluke paragonimus westermani (Paragonimiasis)

paragonimus westermani A. Adult: v Shape rounded 7. 5 -12 mm long X 4 -6 mm wide & 3. 5 -5 mm deep. v wave ceca. v Oral sucker & acetabulum almost equal in size. v Testes slightly branched parallel to each other. v Ovary single, anterior to testes.