LAB 5 Cellular Respiration Slide show by Kelly

LAB 5 Cellular Respiration Slide show by Kelly Riedell/Brookings Biology http: //www. phschool. com/science/biology_place/labbench/lab 5/process. html

LAB SUPPLIES • • • 2 plastic trays (DOUBLE) 2 thermometers 6 glass vials (#1 - #6) with weighted bottoms Food color Rayon balls Cotton balls Dropper/pipetters 18 metal washers Graduated cylinder • • • 2 timers 6 weigh boats Glass beads KOH Masking tape

C 6 H 12 O 6 + 6 O 2 → 6 H 2 O + 6 CO 2 • KOH turns CO 2 gas into a solid • So changes in volume will be due to ? http: //www. phschool. com/science/biology_place/labbench/lab 5/respwork. html

CONTROL VARIABLES • If you want to control variables the volume of the contents in the vials must start the SAME • How can you measure the volume of an irregularly shaped object, like peas?

SET UP RESPIROMETER Add stopper with graduated pipette NON-absorbant (RAYON) protects organism from contact with KOH http: //www. phschool. com/science/biology_place/labbench/lab 5/assemble. html Be careful not to poke into peas Organism Absorbant cotton in bottom / KOH added

Make another set 1, 2, 3 will go in room temp; 4, 5, 6 will go in 10° water http: //www. phschool. com/science/biology_place/labbench/lab 5/assemble. html

See how the pipette is labeled. Distance between numbers =? 0. 1 m. L Each line = ? 0. 01

• Place respirometers in water bath with tips of pipettes resting on a masking tape sling above the water level. • Wait 15 MINUTES for temperature to equilibrate. • Add a drop of FOOD COLOR into the tip of each pipette before lowering them into water. http: //www. phschool. com/science/biology_place/labbench/lab 5/measure. html

• Lower the pipettes into water • Quickly align the tubes so you can read the numbers • Wait 5 MINUTES • Record level of water in each pipette and start timer • Take readings from each respirometer at 5 minute intervals for 20 minutes • REPEAT with 2 nd set of respirometers in ice water http: //www. phschool. com/science/biology_place/labbench/lab 5/measure. html

(Initial reading at time 0) - (reading at time X) 0. 90 25° 10° 0. 92 0. 02 0. 91 0. 85 0. 79 0. 71 0. 65 Peas difference – beads difference 0. 93 0. 06 0. 12 0. 04 0. 10 0. 90 0. 87 0. 03 0. 06 0. 01 0. 04 0. 20 0. 26 0. 18 0. 24 0. 83 0. 81 0. 10 0. 12 0. 08 0. 10

http: //www. phschool. com/science/biology_place/labbench/lab 5/quiz. html

HOW DOES TEMPERATURE AFFECT THE RESPIRATION RATE IN AN ORGANISM? Cold blooded (Ectothermic) http: //www. lbreptileexperience. com/adventures. html Warm blooded (Endothermic) http: //www. uselessgraphics. com/rats. htm

(Initial reading at time 0) - (reading at time X) 0. 90 25° 10° 0. 92 0. 02 0. 91 0. 85 0. 79 0. 71 0. 65 Peas difference – beads difference 0. 93 0. 06 0. 12 0. 04 0. 10 0. 90 0. 87 0. 03 0. 06 0. 01 0. 04 0. 20 0. 26 0. 18 0. 24 0. 83 0. 81 0. 10 0. 12 0. 08 0. 10