KULTUR BAKTERI ISOLASI DAN INOKULASI KULTUR BAKTERI Used

KULTUR BAKTERI -ISOLASI DAN INOKULASI-

KULTUR BAKTERI • Used to grow bacteria • Enrich the numbers of bacteria • Select for certain bacteria and suppress others • Differentiate among different kinds of bacteria

PURE CULTURE • Mikrobia yang dipelajari harus dalam bentuk kultur murni • Strain : kumpulan sel kultur murni yang berasal dari satu sel tunggal • Untuk memperoleh kultur murni maka harus bekerja dengan Teknik Aseptis • Semua bahan dan alat yang digunakan harus steril

PURPOSE OF CULTURING • • Isolation Menyuburkan bakteri To create antigens for laboratory Uji Sensibilitas Uji Aktivitas Antibakteri Estimate viable counts Maintain stock culture

METHODS OF ISOLATION OF PURE CULTURE • SURFACE PLATING • ENRICHMENT MEDIUM • SELECTIVE MEDIUM • INDICATOR MEDIUM

METHODS TO ISOLATE THE BACTERIA • • • STREAK CULTURE POUR PLATE SPREAD PLATE STAB LIQUID CULTURE SPECIAL METHODS FOR ANAEROBIC CULTURES

STREAK PLATE METHOD

SLANT OBSERVATION

STAB CULTURE • Puncturing sutable medium such as nutrient agar, gelatin • Observe gelatin liquefaction • Preserving the stock culture

LIQUID CULTURING Liquid cultures are done in: • Tubes • Bottles • Flasks (labu) • Blood culture • Water Analysis

BROTH MEDIUM

LIQUID MEDIA • Easiest to prepare and use • Good for growing quantities of microbes needed for analysis of experiments • Unless inoculated with pure culture cannot separate different organisms

HOW TO INOCULATE A CULTURE PLATE • Plate : providege large surface for isolation and observation of colonies • Using sterile loop or sterile swab streak your sample on the petri plate • Important let your sterilized loop cool before you pickup your sample

COLONIES – MAKE A OBSERVATION • • SHAPE SIZE ELEVATION EDGE SURFACE OPACITY CONSISTENCY

SWEEP PLATE METHOD

ANAEROBIC BACTERIAL ISOLATION • • Desicator Candle Jar Anaerobic Jar Gas Pack

TERIMA KASIH