KULTUR BAKTERI ISOLASI DAN INOKULASI KULTUR BAKTERI Used
KULTUR BAKTERI -ISOLASI DAN INOKULASI-
KULTUR BAKTERI • Used to grow bacteria • Enrich the numbers of bacteria • Select for certain bacteria and suppress others • Differentiate among different kinds of bacteria
PURE CULTURE • Mikrobia yang dipelajari harus dalam bentuk kultur murni • Strain : kumpulan sel kultur murni yang berasal dari satu sel tunggal • Untuk memperoleh kultur murni maka harus bekerja dengan Teknik Aseptis • Semua bahan dan alat yang digunakan harus steril
PURPOSE OF CULTURING • • Isolation Menyuburkan bakteri To create antigens for laboratory Uji Sensibilitas Uji Aktivitas Antibakteri Estimate viable counts Maintain stock culture
METHODS OF ISOLATION OF PURE CULTURE • SURFACE PLATING • ENRICHMENT MEDIUM • SELECTIVE MEDIUM • INDICATOR MEDIUM
METHODS TO ISOLATE THE BACTERIA • • • STREAK CULTURE POUR PLATE SPREAD PLATE STAB LIQUID CULTURE SPECIAL METHODS FOR ANAEROBIC CULTURES
STREAK PLATE METHOD
SLANT OBSERVATION
STAB CULTURE • Puncturing sutable medium such as nutrient agar, gelatin • Observe gelatin liquefaction • Preserving the stock culture
LIQUID CULTURING Liquid cultures are done in: • Tubes • Bottles • Flasks (labu) • Blood culture • Water Analysis
BROTH MEDIUM
LIQUID MEDIA • Easiest to prepare and use • Good for growing quantities of microbes needed for analysis of experiments • Unless inoculated with pure culture cannot separate different organisms
HOW TO INOCULATE A CULTURE PLATE • Plate : providege large surface for isolation and observation of colonies • Using sterile loop or sterile swab streak your sample on the petri plate • Important let your sterilized loop cool before you pickup your sample
COLONIES – MAKE A OBSERVATION • • SHAPE SIZE ELEVATION EDGE SURFACE OPACITY CONSISTENCY
SWEEP PLATE METHOD
ANAEROBIC BACTERIAL ISOLATION • • Desicator Candle Jar Anaerobic Jar Gas Pack
TERIMA KASIH
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