Kronlab Glass Columns high quality glass columns for
Kronlab Glass Columns • high quality glass columns • for lab scale to process scale • solvent resistant and/or biocompatible • designed for low, medium and high pressure column chromatography The Nest Group, Inc. Slide 1
Product Lines • Eco • Bio. Cart – lab scale – low / medium pressure – lab scale to prep – glass cartridges – medium to high pressure • ECOPLUS – extended pressure rating up to 80 bar – lab scale to prep • Classic – lab scale – medium pressure – conical tube The Nest Group, Inc. Slide 2 • Pilot – pilot scale to production scale – custom designed – solvent resistant or for aqueous buffers
Pilot Columns for Pilot to Production Scale The Nest Group, Inc. Slide 3
Pilot Columns for Pilot to Production Scale • designed for use in pilot and production scale • designed for use with organic solvents (version SR) or aqueous buffers (version AB) • outstanding flow distribution for a linear flow distribution profile • high precision manufacturing of plungers and distribution plates with CNC milling machines, no injection moulding involved The Nest Group, Inc. Slide 4
Pilot Columns for Pilot to Production Scale • full documentation for validation • full service and support for custom designed columns • materials are in compliance with FDA regulations CFR 21, certified if needed • precise and reliable height adjustment of the piston with hand wheels • mounted either on castors or fixed stand or directly into customer´s plant The Nest Group, Inc. Slide 5
Pilot Columns - Wetted Materials • column tube: calibrated borosilicate glass or stainless steel (steel quality to customer‘s specification); optional acrylic • piston: PE, PTFE, PVDF or stainless steel • seals: EPDM or Kalrez® • frits: PE, stainless steel, others on request The Nest Group, Inc. Slide 6
Pilot Columns - Sizes • columns from 60 mm to 600 mm ID • length on demand The Nest Group, Inc. Slide 7
Pilot Column Design of Flow Distributor flow distribution profile proofed by Bayer AG and Kronlab Gmb. H The Nest Group, Inc. Slide 8
Pilot Column flexible inner diameters PI 450 PI 60 PI 100 PI 140 Flow distribution profile proofed by Bayer AG and Kronlab Gmb. H The Nest Group, Inc. Slide 9 PI 100 Cooling Jacket
Pilot Column flexible column material stainless steel The Nest Group, Inc. glass or acrylic Slide 10
Pilot Column flexible pressure ranges 8, 7 bar 10 bar 1 bar The Nest Group, Inc. Slide 11 50 bar
Pilot Column integration of user demands e. g. rotable frames, integrated level indicator, brackets, selected materials, connectors ………. The Nest Group, Inc. Slide 12
Pilot Columns Custom Design special solutions for individual needs regarding: • materials • design • size • system integration The Nest Group, Inc. Slide 13
Pilot Columns Custom Design • individual feasibility studies and technical drawings • certification • competent guidance throughout the whole project The Nest Group, Inc. Slide 14
Pilot AB - rotatable column id: 200 mm max. bed height: 1600 mm pressure rating: 7 bar specially designed rotatable frame construction The Nest Group, Inc. Slide 15
Pilot SR – solvent resistant technical specifications: column id: max. bed height: pressure rating: 200 and 300 mm 400 mm 1. 5 bar explosion-proof environment with antistatic protection and wheels The Nest Group, Inc. Slide 16
Pilot SR – stainless steel technical specifications: column id: max. bed height: min bed height: pressure rating: sealing: The Nest Group, Inc. 100 mm 1000 mm 500 mm 40 bar Kalrez® Slide 17
Pilot SR – stainless steel technical specifications: column id: max. bed height: min bed height: pressure rating: sealing: The Nest Group, Inc. 150 mm 900 mm 700 mm 50 bar Kalrez® Slide 18
Pilot AB - Reactor id: bed height: pressure rating: frit: 300 mm 0 - 500 mm 1 bar 30 µm (SS 1. 4404) special features: • slurry inlet • integrated magnetic stirrer on cambered bottom frit • piston with scraper • special designed column stand The Nest Group, Inc. Slide 19
Pilot AB -rotatable id: 240 mm bed height: 0 - 270 mm volume: 0 -12 l pressure rating: 3 bar cooling jacket: 15 - 65°C SS - container: 15 l for slurry waste frits: 10 µm PE 5 mm thickness specially designed frit fastening screws to ensure perfect flow distribution The Nest Group, Inc. Slide 20
Pilot Columns stainless steel id: 600 mm max. bed height: 1000 mm (1500 mm) bed volume: 282, 6 l (423, 9 l) pressure rating: 10 bar frits: 10 µm The Nest Group, Inc. Slide 21
Example Pilot Column stainless steel id: 600 mm max. bed height: 1000 mm (1500 mm) bed volume: 282, 6 l (423, 9 l) pressure rating: 10 bar frits: 10 µm designed for repeated packing and unpacking without opening the column The Nest Group, Inc. Slide 22
Solvent Inlet Example Slurry-Inlet through separate slurry pump Slurry-Outlet through central outlet valve Solvent Outlets The Nest Group, Inc. Slide 23
Pilot Columns - References • • • • Abbott Aventis Baxter Hyland Immuno AG Bayer AG Biotest Clariant Genetics Institute Genzyme Honeywell Novartis AG Schering AG Roche Diagnostics etc … The Nest Group, Inc. Slide 24
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