KONIS Manual obtained in a manner to prevent

KONIS Manual

“obtained in a manner to prevent introduction of organisms from the surrounding tissues into the specimen being collected” 무균적 채취 (Aseptic technique) swabs collected at the bedside or in the OR can be aseptically obtained. General guidelines for the collection of optimum wound specimens https: //www. cdc. gov/nhsn/faqs/faq-ssi. html

Wound culture – Superficial wound • Disinfect wound surface with alcohol and then a 10% povidone-iodine solution. Allow disinfectant to dry • Aspirate • Using a 3 to 5 ml syringe and a 22 to 23 gauge needle • If no aspirate is obtained, sterile, non-bacteriostatic 0. 85% saline • Swab • Lift edge of overlying debris (scab), if present. • If exudate or pus is present, sample with swab. In the absence of fluid, swab leading edge of wound. Clinical Microbiology Procedures Handbook, 1992

Wound culture – Deep wound or abscess • Disinfect wound surface with alcohol and then a 10% povidone-iodine solution. Allow disinfectant to dry • Aspirate • Use needle and syringe to aspirate the deepest portion of the lesion, avoiding contamination with wound surface. If performed as a surgical procedure, include a portion of the abscess wall Clinical Microbiology Procedures Handbook, 1992