KirchhoffInstitut fr Physik Biophotonics I W Petrich Slides

Kirchhoff-Institut für Physik Biophotonics I W. Petrich Slides of lecture #5 November 11 th, 2019 http: //www. kip. uni-heidelberg. de/biophotonik/teaching • Lecture „Biophotonics I“ will be credited with 2 CP subject to successfully passing the exam • There will be a written exam („Klausur“) on February 3 rd 2020 • There is NO lecture on December 2 nd, 2019 Prof. Dr. Petrich Biophotonics I (WS 2019/20) 1

Biophotonics I SUMMARY of lecture#4 (4. 11. 2019) Kirchhoff-Institut für Physik c) biochemistry and time resolution (cont. ) I. 2. Microscopy • Biochemical process (e. g. rods): 11 -cis retinal all-trans retinal 0. 5 nm in ~ ps I. 2. 1. setup and principle … Metarhodopsin II ~ ms Magnification of a magnifying glass: … closing Na+/Ca 2+ ion channels hyperpolarization … recovery to initial state ~ 10… 100 ms R • Time resolution High luminance: up to 60 Hz (rods&cones) Low luminance: ≤ 15 Hz, rods faster than cones CIE norm 5033 (1931) http: //www. cie. co. at/cie/ d) attempts towards color metrics e) artificial vision • • external camera plus microelectrode array (localisation: 29 of 30 persons; 20/1260 visual acuity) Implanted sensor plus microelectrode array (localisation: 7 of 9 persons; 20/540 visual acuity) Prof. Dr. Petrich D : optical tube length (typ. : 160 mm) sources: www. microscopyu. com, www. olympusmicro. com Biophotonics I (WS 2019/20) 2

Biophotonics I I. 2. Microscopy Kirchhoff-Institut für Physik Blendenstrahlengang (Pupillenstrahlengang) Lukenstrahlengang brightness field of view source: http: //www. olympusmicro. com Prof. Dr. Petrich Biophotonics I (WS 2019/20) 3

Biophotonics I I. 2. Microscopy Kirchhoff-Institut für Physik www. zeiss. de Prof. Dr. Petrich Biophotonics I (WS 2019/20) 4

Biophotonics I I. 2. Microscopy: definition of resolution limits Prof. Dr. Petrich Biophotonics I (WS 2019/20) Kirchhoff-Institut für Physik 5

Biophotonics I I. 2. Microscopy: definition of resolution limits Kirchhoff-Institut für Physik Abbe Rayleigh Sparrow Prof. Dr. Petrich Biophotonics I (WS 2019/20) 6

Biophotonics I REMINDER: Gaussian beams Kirchhoff-Institut für Physik www. htw-aalen. de Prof. Dr. Petrich Biophotonics I (WS 2019/20) 7