Kegagalan marka Bradbury mengidentifikasi varietas aromatik Pandan Wangi
Kegagalan marka Bradbury mengidentifikasi varietas aromatik Pandan Wangi Sampel aromatik Standar nonaromatik Standar aromatik Gel of the varieties and accessions given in the Table , showing that none have the 8 -bp deletion that has been reported to cause the accumulation of 2 acetyl-1 -pyrroline (2 AP). Khao Dawk Mali 105 (KDM), which carries the deletion, and Calrose, which does not, are shown as controls. KNL, Kai Noi Leung; PSH, Paw San Hmwe; PW, Fitzgerald et al. 2008, Pandan Wangi. Kovach et al. 2009
Usulan mutasi gen badh 2 varietas aromatik Kovach et al. 2008
The genotypes of fragrant and non-fragrant rice varieties (Shi et al. 2008, Sakthivel et al. 2009) Alleles badh 2. 2 badh 2. 7 Sequencedivergences A 7 -bp An 8 -bp deletion in deletion and 3 exon 2 SNPs in exon 7 No Yes No Sakthivel et al. 2009 badh 2. 2/ badh 2. 7 No Rice varieties Fragrant No 3037, Ballila, Wuyunjing, 02428, Laolaiqing, Miyang 46, Liaojing 5, Zhenzhuai, Baxizaodao, Dullar No No Wuxiang 9915, Xiangjing 111, Zhenxiangjing 5, Wuxiangjing 9, Xiangjing 02– 5855, Xiangjing 49, Gehuxiangjing, Guanglingxiangjing, Xiangjing 111/C 9083, Wuxiang 99 -8, Wuxiangjing 14, Suxiangjing 1 Yes Suyunuo, Wuxiangjing, Pangxiegu, Xiangxuenuo, Xiangjing. T 37, Wuxiang 075, Xiangjing 20– 18, Basmati 385, Basmati 370, Ganxiangnuo, Meiguomolixiang, Guanglingxiangnuo Yes No Tarunbhog, Ganjeikalli, Bishnubhog, Bansphool A and Adamchini Yes
PCR DENGAN VARIASI PRIMER BARDBURY I = Padi aromatik P 1, P 2, dan Rl tidak teramplifikasi (seharusnya teramplifikasi) II = Padi aromatik P 1, P 2, dan RL teramplifikasi (seharusnya tidak teramplifikasi) III = Primer lengkap ESP INSP IFAP EAP bp EAP IFAP EAP 1000 500 I * 100 C P 1 P 2 Padi Nonaromatik C = Ciherang II * Rl M C P 1 P 2 III Rl M C P 1 P 2 Rl M Padi Aromatik P 1 & P 2 = Pandan Wangi 1 & 2 Rl = Rojo lele M = Mentik Wangi
AMLIFIKASI DNA MENGGUNAKAN PRIMER BRADBURY ESP INSP NA 355 bp A F 1 580 M Nonaromatik (NA) IFAP Delesi EAP 580 355 Aromatik (A) 355 355 257 580 bp Kelompok 2 : Pandan Wangi, Rojolele, Pulu Mandoti , Gilirang, dan Pare Kembang INSP Delesi Kelompok 1 : Mentik Wangi, Gunung Perak ESP INSP 257 bp Aromatik IFAP EAP Nonaromatik Aromatik IFAP ESP=External Sense Primer IFAP=Internal Fragrant Antisense Primer INSP=Internal Non-Fragrant Sense Primer EAP=External Antisense Primer
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