KASAHARA Industry Co Ltd OKHOTSK CALCIUM Calcinated Scallop

KASAHARA Industry Co. , Ltd. OKHOTSK CALCIUM “Calcinated Scallop Shell Calcium” Complying with the “Japanese Standards of Food Additives: Calcinated Shell Calcium” mentioned in Article 21 of the Food Sanitation Act which is supervised by the Ministry of Health, Labour and Welfare 1

About OKHOTSK CALCIUM KASAHARA Industry Co. , Ltd. 1　Food Additive ２　Mechanism of Okhotsk Calcium ３　Sterilizing and Antibacterial Effectiveness ４　Antifungal Effectiveness ５　Deodorizing Effectiveness ６　A Variety of Our Products The structure of Calcinated Scallop Shell Calcium 2

KASAHARA Industry Co. , Ltd. １ The Okhotsk Calcium is a food additive. 　 The raw material of “Okhotsk Calcium” is the shells of the scallops caught around Hokkaido. The shells are heated over on high heat of about 1, 100 degrees and turns into the powder containing Calcinated Calcium. It is the multifunctional product made from the 100% natural material. The powder complies with the “Japanese Standards of Food Additives: Calcinated Shell Calcium. ” 3

Scallop Catch The scallop catch in the Okhotsk Sea, which our factory is on, is 300, 000 ton, 150, 000 ton of which is used for our products. Fisheries in Hokkaido Scallop Catch by regions Throughout Hokkaido: About 400, 000 ton Throughout Japan: About 500, 000 ton Source: Department of Fisheries and Forestry, Hokkaido Government 4

KASAHARA Industry Co. , Ltd. ２　Mechanism of Okhotsk Calcium The main component of the scallop shell is calcium carbonate. Calcinated on high heat, it is decomposed into calcium oxide and carbon dioxide. The calcium oxide is called Okhotsk Calcium. Ca. CO 3 High Heat CO 2 calcium carbonate (cracked scallop shells) Ca. O carbon dioxide Okhotsk Calcium (calcium oxide) Reacting with water, calcium oxide (Okhotsk Calcium) turns into calcium hydroxide. The solution is the strong-alkaline one with more than PH 12. The strong-alkaline solution has the sterilizing and deodorizing effectiveness and decomposes bacteria such as Escherichia coli and O 157, viruses, and acetic acid and acetaldehyde which are the origin of the odor, etc. Ca. O Okhotsk Calcium (calcium oxide) H 2 O Chemical Reaction Water Formation of Radical Ca(OH)2 calcium hydroxide ※ Radical: atoms, molecules or ions with unpaired electrons 5 　

KASAHARA Industry Co. , Ltd. ３ -1　Sterilizing, Antibacterial Effectiveness ①Bacteria　 Calcinated Shell Calcium Solution・Concentration Saturated Solution, 25℃ ca. 0. 1% Concentration Bacteria Count Before Sterilization (cfu/m. L) Time Spent for Sterilization Count After Sterilization (cfu/m. L) Test Organization Escherichia coli 3. 2× 10＾ 5 10 minutes 30 or less than 30※ Salmonella 2. 3× 10＾ 5 30 or less than 30※ Tokyo Metropolitan Food Technology Research Center Pseudomonas aeruginosa 3. 2× 10＾ 5 30 or less than 30※ Staphylococcus aureus 2. 7× 10＾ 5 40 Escherichia coli Serotype O 157: H 7 4. 0× 10＾ 5 10 minutes Less than 10※ Japan Food Research Laboratories Methicillinresistant Staphylococcus aureus(MRSA) 1. 6× 10＾ 5 3 hours Less than 10※ Kyoto Biseibutu Kenkyusho (Biken) 6

KASAHARA Industry Co. , Ltd. 3 -2　 Sterilizing, Antibacterial Effectiveness Calcinated Shell Calcium Solution・ Concentration Saturated Solution, 25℃ ca. 0. 1% Concentration ② Virus 　Calcinated Shell Calcium Solution・ Concentration Saturated Solution, 25℃ ca. 0. 1% Concentration Bacteria Count Before Sterilization (cfu/m. L) Time Spent for Sterilization Count After Sterilization (cfu/m. L) Test Organization Vibrio parahaemolyticus 1. 2× 10＾ 5 5 minutes 　Less than 10※ Kyoto Biseibutu Kenkyusho (Biken) Legionella 0. 82× 10＾ 5 10 minutes Less than 10※ Virus Infectivity Titer Before Sterilization Time Spent for Sterilization Infectivity Titer After Sterilization Test Organization 7. 0 10 minutes 　Less than 1. 5※ Japan Food Research Laboratories (log. TCID 50/ml) Norovirus (feline calicivirus used as a substitute) (log. TCID 50/ml) 7

KASAHARA Industry Co. , Ltd. 4 -1　 Antifungal Effectiveness ① The antipropagation of fungus Calcinated Shell Calcium Fungus Aspergillus ｂrasiliensis Test Procedure Add a given amount of Calcinated Shell Calcium powder to potato dextrose broth*. *It is the broth produced by adding potato extract, glucose and agar to purified water. It is adequate for the propagation of fungus. Inoculate some petri dishes (about 20 ml) with about 10 fungal spores. They are cultured at a room temperature and the propagation of fungus is observed with eyes at some intervals. Days ２ ５ ７ ０ｇ 0. 3ｇ/200 ml （ca. 0. 15％） 3ｇ/200 ml （ca. 1. 5％） 8

KASAHARA Industry Co. , Ltd. 4 -2　 Antifungal Effectiveness Fungus Test Procedure Add a given amount of Calcinated Shell Calcium powder to potato dextrose broth*. *It is the broth produced by adding potato extract, glucose and agar to purified water. It is adequate for the propagation of fungus. Inoculate some petri dishes (about 20 ml) with about 10 fungal spores. They are cultured at a room temperature and the propagation of fungus is observed with eyes at some intervals. Calcinated Shell Calcium Days ２ Aspergillus orｙzae ５ ７ ０ｇ 0. 3ｇ/200 ml （ca. 0. 15％） 3ｇ/200 ml （ca. 1. 5％） 9

KASAHARA Industry Co. , Ltd. 4 -3　 Antifungal Effectiveness Fungus Test Procedure Add a given amount of Calcinated Shell Calcium powder to potato dextrose broth*. *It is the broth produced by adding potato extract, glucose and agar to purified water. It is adequate for the propagation of fungus. Inoculate some petri dishes (about 20 ml) with about 10 fungal spores. They are cultured at a room temperature and the propagation of fungus is observed with eyes at some intervals. Calcinated Shell Calcium Days ２ Penicillium camemberti ５ ７ ０ｇ 0. 3ｇ/200 ml （ca. 0. 15％） 3ｇ/200 ml （ca. 1. 5％） 10

KASAHARA Industry Co. , Ltd. 4 -4　 Antifungal Effectiveness ②　Results Calcinated Shell Calcium Solution・ Concentration Saturated Solution, 25℃ ca. 0. 1% Concentration Fungus Count Before Sterilization (cfu/m. L) Time Spent for Sterilization Count After Sterilization (cfu/m. L) Test Organization Aspergillus ｂrasiliensis 9. 8× 10＾ 4 3 days 10 or less than 10※ Tokyo Metropolitan Food Technology Research Center Aspergillus orｙzae 6. 5× 10＾ 4 3 days 10 or less than 10※ 11

KASAHARA Industry Co. , Ltd. ５　 Deodorizing Effectiveness Results The odor of rotten eggs Hydrogen sulfide Gas concentration at the time when only the gas is injected Gas concentration at the time when calcinated calcium solution is injected Test Organization: Japan Food Research Laboratories (Injecting the gas into a bag and measuring by the detector tube method) 　 The odor of rotten onions Methyl mercaptan The odor of vinegar Acetic acid The odor of sweaty socks Isovaleric acid 時間(min) The odor of urine Ammonia 時間(min) The odor of rotten fish Trimethylamine Stimulating, grassy odor Acetaldehyde Strongly effective against, especially, acid odor 12

KASAHARA Industry Co. , Ltd. 6 -1　 A Variety of Our Products For Home Liquid-type Powder-type Examples of how they are used ●　 For deodorizing kitchens, rooms, etc. ●　 For deodorizing cars and removing bugs on windshield ●　 For easing pets’ skin disease and eliminating their odor ●　 For sterilizing fresh vegetables and removing the residual agricultural chemicals ●　 For eliminating the odor and the bacteria of hang-to-dry indoors’ clothes ●　 For sterilizing the playground for children 13

KASAHARA Industry Co. , Ltd. 6 -1　 A Variety of Our Products For Business Hotatect Ｗ Products by volume 　 380 ml 1000 ml 5000 ml 20ℓ Examples of how they are used ●　For sterilizing the places where you deal with foods such as kitchens ●　For deodorizing and sterilizing rooms, tables, corridors, etc. ●　For deodorizing bathrooms and deodorizing and sterilizing garbage dumps They are used for deodorizing and sterilizing the various places or things. 14

KASAHARA Industry Co. , Ltd. 6 -2　A Variety of Our Products For Industrial Materials ●　Adding antibacterial and deodorizing effectiveness e. g. )　Producing wall materials with antibacterial, deodorizing and antifungal effectiveness by kneading calcinated calcium into PP sheet e. g. )　Coating industrial materials with calcinated calcium by applying the technology of silkscreen printing e. g. )　Producing PP cutting boards to eliminate the various odors Company N produces wall materials with antibacterial and deodorizing effectiveness by kneading calcinated calcium into PP sheet Insole 15

KASAHARA Industry Co. , Ltd. 6 -3　 A Variety of Our Products For Facilities ●　 Used as a substitute for hypochlorous solution, etc. e. g. )　 At the washing line for eggs of poultry farms e. g. )　 At the dissection rooms of medical universities (for antifungus and deodorization) e. g. )　 At the long-term care facilities (for sterilization and deodorization and avoiding nosocomial infection) e. g. )　 For washing and sterilizing cut vegetables and keeping them fresh A midsize poultry farm (Shizuoka Prefecture) Our solution is used as a substitute for hypochlorous solution at a sterilizing and washing line. 16

KASAHARA Industry Co. , Ltd. 6 -4　 A Variety of Our Products For Foods ●　 Used as food additives e. g. )　 A substitute for lye water which is used for noodles 　　　　　→ It is called lye water-free noodle. e. g. )　 Binding agent for meat　→ It is used for cube-shaped steak, etc. e. g. )　 Coagulant for konjac e. g. )　 Health food, Calcium supplement 　 Lye water-free noodle The solution is used as a substitute for lye water. 17

KASAHARA Industry Co. , Ltd. Thank you for your attention. KASAHARA Industry Co. , Ltd. Tokyo Office 3 F Tsukasa Building, 2 -15 -4, Uchikanda, Chiyoda-ku, Tokyo, 101 -0047 Japan TEL: +81 -3 -3256 -2426 FAX: +81 -3 -3256 -3560 18