Kansas Harmful Algal Bloom HAB Monitoring Program Laboratory

Kansas’ Harmful Algal Bloom (HAB) Monitoring Program Laboratory Analysis of Cyanotoxins 2016 Stakeholder’s Meeting January 7, 2016 Presented by: Tony Stahl, KDHE

KDHE’s Analytical Process for Cyanotoxins Measurement Analytical Support – Microscopic Examination Typical Cyanotoxin-Producing Cyanobacteria (blue-green algae) Laboratory Methods Employed for Cyanotoxins Measurement Our Mission: To protect and improve the health and environment of all Kansans.

Sample collection – Laboratory Analysis – Our Mission: To protect and improve the health and environment of all Kansans. Results Posted Online

Microscopic Examination • Identification and enumeration (cell count) estimates of number of freshwater phytoplankton, including, but not limited to cyanobacteria, in water samples collected from lakes, wetlands, rivers and streams using microscopic techniques Microcystis sp. colony Our Mission: To protect and improve the health and environment of all Kansans. Cylindrospermopsis sp.

Information obtained from microscopic examination can be used to make an informed decision on the type of bioassay technique which should be employed. Current analytical capacity to measure Adapted and modified from: A guide to their public health consequences, monitoring and management (WHO, 1999) Our Mission: To protect and improve the health and environment of all Kansans.

• A laboratory can easily establish, in times of tight budgets with little or no access to adequate equipment and expertise to establish physicochemical analysis (e. g. , LC/MS/MS detection), or little means of subcontracting analysis. • ELISAs techniques typically have the ability to detect toxins at low levels for surface and drinking waters. • Popular screening assays for Cyanobacterial taxa dominate waters due to very good cross-reactivity with multiple cyanotoxins congeners. Our Mission: To protect and improve the health and environment of all Kansans.

Toxin analyses • Perform the enzyme-linked immunosorbent assay (“ELISA”) test for determination of microcystins (using ENVIROLOGIX test kit and procedure) • Perform the “ELISA” test for determination of microcystins (using ABRAXIS Ohio EPA ADDA by ELISA test kit and procedure) or cylindrospermopsin (ABRAXIS, ELISA kit) using a microtiter plate and microplate reader Our Mission: To protect and improve the health and environment of all Kansans.

Our Mission: To protect and improve the health and environment of all Kansans.

www. kdheks. gov Our Mission: To protect and improve the health and environment of all Kansans.