JAXA CBEF ESA Biolab ESA EMCS ESA Kubik

JAXA CBEF ESA Biolab ESA EMCS ESA Kubik Techshot NASA Bioculture ADSEP/C NASA System ell Cult BSTC Techshot Bio. Serve MOBIAS CHab FPA /GAP BCA GAP H/W Availability On ISS In production Req ISS Cert. In Re. Cert ISS Cert. Adherent Cells Non-Adherent Cells 1 Y Y Y Y N Y Gas-Permeable Bag 2 Y Y Y N Y Hollow Fiber Bioreactor Rigid Cell Chamber Fluid Loop Gas Perfusion User-Defined EUE Y Y Y N Y N Y Y N N N Temperature Control (o. C) 15 to 40 18 to 40 6 to 38 4 to 39 4 to 50 10 to 37 CGBA 4 to 37 Insert CO 2 Control (%) Humidity Control (%) Automated Medium Circulation Automated/Manual Sampling /Injection Automated/Manual Injection 0 to 10 20 to 80 0 to 5. 5 60 to 90 0 to 5. 5 50 to 90 Passive Y Y Two chambers Ambient to 5 Ambient to 42 5 Passive Passive Y Y Y N A/M A/M A/M Centrifuge 1 rotor 0. 1 2 rotors 1 rotor to 2 0. 001 to 2 0. 2 to 2 ISS Cert. Y Y N Y Y N N Maybe Y N N N N Maybe N Y CGBA Insert Possible Passive Passive Y Y N N Y M N A M A/M M Y M N A Possible N N N N Temp/CO 2/RH Sensors Power to EUE Data and Commanding Video T/C/RH Y Y Y T N N N T/C N/A Y Can Accomodate T N/A D N T/C N Y N N/A D Y N/A N/A N/A 3 Y Lighting Y Y Y N Can Accomodate N N N Y Y

Priority Research Areas NASA BSTC Techshot MOBIAS Bio. Serve CHab Bio. Serve FPA /GAP Bio. Serve BCA JAXA CBEF ESA Biolab Virulence: Host/Pathogen Mechanism Y* Y* Y Y Y* Tissue Engineering Y* Y Y Cell Specialization Y (CEU 1, CEU 2*) Y* Y* Y* Y Y Novel Therapeutics Y (CEU 1, CEU 2) Y Y* Y* Y Y Acute Y Y Y Long Term Y Y Y N N Auto /Manual Auto Priority Capabilities ESA EMCS Techshot NASA Bioculture ADSEP/Ce ESA Kubik System ll Cult Auto/Manual Auto/Manual Auto /Manual Mix cultures/co-culture Y Y Y N Haz Containment Y Y Y N Auto Injection Auto/Manual Auto Manual Auto /Manual T/G/RH T T/G T T Mass Transfer H/W Depend N Y Y N N N Low Shear Environment (Fresh Medium Addition) H/W Depend N Y N N N/A N N N Auto /Manual N/A 4 Sampling Environmental Control (Temp [T], Gas [G], , Relative Humiidty [RH]) p. H Sensor Fresh Medium Addition Provide "N" for Statistics Variable g-force Static (no continuous medium flow) Continuous Medium (CM) Flow/Perfusion (P) Auto N/A Auto Manual Auto 10 (20 if multiplexed) 3 4/frame 32 total 12 min. More for less than 50 ml bags 6 min 6 or more dependent on FPA type used 6 (static) 4 (Cent) 6/Cent 4/Cent 16 -40 (static) 12 (Cent) Y Y N N N N H/W Depend Y Y N Y Y Y N CM/P N CM CM N N CM; H/W Depend Volumes H/W Depend 1. 5 ml to 7 ml Vessel 50 ml Vessel 30 ml Bag Depend. Container Depend. 32 ml bag Multi-Wells H/W Depend Biochamber specific N N N Y Y Can Accommodate N N N Y N N Supports Cell Imaging On. Orbit *Priority hardware for the research area; H/W = hardware