Isotonix Immune Supporting Your Innate Immunity Mark Lange

® Isotonix Immune Supporting Your Innate Immunity Mark Lange, Ph. D Director, Quality Control

Disclaimer These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

The Hygiene Hypothesis §The shift from dirty to sterile has changed our immune response. §The idea that sterile is good, sterile is healthy, is being questioned.

Our Urban Lifestyle Attempts to Eliminate Naturally-Occurring Microbes, Yeasts and Molds

Exposure to Dirt/Microbes Might Actually Upregulate Immune Response § Children going to daycare have fewer asthma and allergies as they grow up. § Children living on farms less likely to have asthma and allergies. § Growing up in large family gives exposure to viruses and microbes. § Breast-fed babies have fewer allergies than bottle-fed.

Is Getting Dirty the Best Way to Support Natural Immune Response?

How Your Immune System Works § Creates a barrier that prevents bacteria and viruses from entering body. § Your innate (meaning “present from birth”) immune system detects and destroys without needing to be trained to identify the intruders. (FAST) § Your adaptive immune system learns. (SLOWER)

Cells of the Innate Immune System §Neutrophils are very abundant. §Attack in large numbers. §They are the first responders; within minutes. §Survive only 1 -2 days.

Cells of the Innate Immune System §Macrophages (“big eaters”) §Hang out in lung, gut, mucosal tissue. §Either “eat” (phagocytize) invaders or §Give an external oxidative burst to kill invaders.

Complement § Is like a chemical red flag, marking pathogens as being “non-self. ” § Acts as an attractant to innate immune cells, such as neutrophils. § Complement also enhances the binding of the pathogen for phagocytosis, enhancing the antimicrobial activities of neutrophils.

Supporting a Healthy Immune System

Wellmune® § Derived from a proprietary strain of yeast. § Generally Recognized as Safe (GRAS) and non-allergenic. § Effectiveness proven in clinical trials. § Source of beta glucans, which act as complement to neutrophils.

How Wellmune® Works After ingestion, Wellmune is absorbed in the GI tract through Peyer’s patches.

Macrophages Digest Wellmune Enzymes Wellmune Active Fragments Slowly released

Engulfing a Bacterium Beta Glucan Fragment Receptor (CR 3) Neutrophil Bacterium flagged with complement Captured Bacterium

Two More Immune-Supporting Ingredients § Zinc § Phagocytosis § Generation of oxidative burst § Vitamin C § Protects cell membrane from oxidative burst

Wellmune Improves Mood State § S. M. Talbott and J. A. Talbott. Baker's Yeast Beta-Glucan Supplement Reduces Upper Respiratory Symptoms and Improves Mood State in Stressed Women J Am Coll Nutr. August 2012 vol 31 no. 4 pp 295 -300. § S. M. Talbott and J. A. Talbott. Effect of BETA 1, 3/1, 6 GLUCAN on upper respiratory tract infection symptoms and mood state in marathon athletes Journal of Sports Science and Medicine (2009) 8, pp 509 -515. § Placebo controlled studies showing decreased confusion, fatigue, tension, anger and increased vigor after stress.

Isotonix® Immune Benefits § Boosts the body’s natural immune response. § Clinical trials show Wellmune® promotes a healthy immune system. § Promotes beneficial activity of macrophages. § Promotes healthy energy levels and vitality. § Improves mental clarity.

Frequently Asked Questions § Wellmune is made from yeast. Will it cause yeast overgrowth in my body? § What is the difference between Isotonix Immune and the old Immune Maximum Defense Formula? § I already take Isotonix OPC-3. What benefit does Isotonix Immune provide? § Can children take it? § If I take more, will it make my immune system even stronger?