Isolation and Characterization of Bacteria Producing Substances Against
Isolation and Characterization of Bacteria Producing Substances Against Gram Positive Bacteria 報告學生:鄭燕如 張如慧 報告日期: 2003/12/29
材料I 第四組及淡水的土樣 n 革蘭氏陽性菌株 Bacillus cereus Staphylococcus aureus subsp. aureus n 9 m. L 0. 85% Na. Cl、100 ppm cycloheximide n 2, 5, 7, 10%Na. Cl n glucose、xylose、mannitol、lactose、fructose、 arabinose n
材料II n Citrate n Urea broth n Mac. Conkey agar n Mannitol Salt Agar n SIM agar n Voges-Proskauer n 1/3 TSA
實驗流程-III<傳統生化測試> n Gram stain n Spore stain n Carbohydrate fermentation n 2, 5, 7, 10%Na. Cl n Citrate uilization test n Urease test n Mac. Conkey agar n Mannitol Salt Agar
實驗流程-IV<傳統生化測試> n Nitrate reduction test n Catalase test n Oxidase test n Voges-Proskauer test n Indole production test n Triple sugar-iron agar test n SIM agar
分類樹一. G(-) ‚rod且catalase test為正反應 Bdellovibio spp. Azospivillum spp. Halobacterium spp. Acetobacter spp. Legionella spp.
分類樹一. Oxidase (+) (-) Bdellovibio spp. Acetobacter spp. Azospirillum spp. Legionella spp. Halobacterium spp. Legionella spp. Urease
分類樹一 urease (+) Bdellovibio spp. Azospivillum spp. Halobacterium spp. Acetobacter spp. (-) Bdellovibio spp. Acetobacter spp. Legionella spp Glucose fermentation
分類樹一 Glucose fermentation H. volcanii H. saccharovorum H. vallismortis Acetobacter paseurianus A. aceti A. liquefocieus A. hansenii Bdellovibio spp.
分類樹一 Nitrate reduction (+) (-) H. saccharovorum A. aceti H. vallismortis A. liquefocieus H. volcanii 外觀形態
分類樹一 Pale color Paink color A. aceti較無移動性(a 6) H. volcanii A. liquefocieus較具移動性(a 3)
分類樹二 Endospore-rod (+) Bacillus spp. Sporolactobacillus spp. Clostridum spp. Desulfotomaculum spp. Oscillospira spp. (-) Sporosarcina spp. Planococcus spp. Lactobacillus spp. Kurthia spp.
分類樹二 catalase (+) Bacillus spp. (-) Sporolactobacillus spp. Clostridum spp. Desulfotomaculum spp. Oscillospira spp.
分類樹二 xylose (+) B. alcalophilus B. circulans B. lentus B. macerans B. macquarinsis B. marinus B. megaterium (-) B. thuringiensis B. brevis B. globisporus B. sphaericus B. insolitus B. laterosporus B. pantothrnticus B. firmus B. schlegelii B. stearothermophilus
分類樹二 (+) b 1 d 3 B. Megaterium B. Sphaericus B. Stearothermophilus B. thuringiensis glucose (+) b 1 d 3(-) citrate b 2 (-) B. Brevis B. Insolitus B. Laterosporus B. Marinus B. Pantothrnticus B. schlegelii B. sphaericus glucose (-) b 2
分類樹二 b 1 glucose d 3 (-) (+) B. Megaterium B. Sphaericus B. Stearothermophilus B. thuringiensis glucose (+) b 2 (-) B. Insolitus B. schlegelii
表二. 生化測試-II 測試項目 citrate mannitol lactose fructose arabinose urea broth a 3 + - - a 6 + - - b 1 + - - b 2 - + - - d 3 + + - -
Gram stain a 3 G(-) a 6 G(-) b 1 G(+ ) b 2 G(+)
Gram stain Spore stain d 3 G(+) d 3
Spore stain a 3 a 6
Spore stain b 1 b 2
MSA test
參考文獻 n Bergey‘s manual of systematic bacteriology volume 1 n Bergey‘s manual of systematic bacteriology volume 2
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