Irrigation Effects 1 st Trial Vs Peachtree Borer

Irrigation Effects, 1 st Trial Vs. Peachtree Borer • Treatments: no irrigation, Irrigation (3 X per wk over 2 wks), Barricade, chlorpyrifos (Lorsban) Irrigation Effects Spring 2013 Figure 1 % Infested Lack of irrigation = fail With irrig – suppression Barricade trt = Lorsban 100 A 80 60 B 40 20 BC C 0 Barricade Irrig Lorsban Treatment No Irrig

Effect of Application Method, 1 st Trial Vs. Peachtree borer • Treatments: commercial nematodes with boom sprayer, handgun, trunk sprayer, non-treated control. In vivo nematodes with watering can, chlorpyrifos (pos control); E -nema with handgun • Apply in fall, assess PTB infestation in spring % PTB Infestation Assessed Spring 20113 Byron GA 80 60 A 40 A A 20 A A vi v Lo o rs ba n Tr un k In n an dg u em En H ol tr on C a. H 0 Bo Figure 2 A A om • High variation – no sig Treatment differences 100 % Infestation • 7 trees per rep 3 reps per treatment Treatment

Results of st 1 C ol tr C on B 2 % B- B 1 0. 5 eo qu A C % C Treatment Survival of G. mellonella after 30 min A 100 80 60 40 20 0 A B C on tr ol 2% B- 1% C B- 0. 5% B- us C qu eo A % Survival • After 1 h all treatments failed • Barricade protected nematodes in single spray up to 30 min A B us tr ol on C Treatment Figure 3 100 80 60 40 20 0 % % Survival B % % 5% 0. B- eo qu B B B 2 B B 1 100 80 60 40 20 0 A Survival of G. mellonella after 15 min A us % Survival of G. mellonella after 0 min Trial Treatment

Field Trial Results vs. Lesser Peachtree borer: 2. 5 A A 2 B 1 0. 5 B B 0 -0. 5 -1 Barr 2% Barr. Full Control Treatment Nufos Nem. Only % Control # Live 1. 5 100 90 80 70 60 50 40 30 20 10 0 A A A B Barr 2% Barr. Full Nufos Treatment Nem. Only • Nematodes + Barricade at full and 2% rates provided control equal to Nufos (Lorsban) Figure 4