INVENTORS Nor Amirra Abu Nayan Chan Kok Yan
INVENTORS Nor Amirra Abu Nayan, Chan Kok Yan, Nurul Ain Harmiza Abdullah, CONTACT DETAILS Email: ainharmiza@uunimap. edu. my H/P no. : 013 -3600043 UTILIZATION OF THE WASTEFUL SKIM LATEX SERUM FOR ENZYME PRODUCTION INTRODUCTION Biomass-Product-Remediation Profile 14. 0 0. 20 12. 0 0. 15 10. 0 8. 0 0. 10 6. 0 4. 0 0. 05 2. 0 0. 00 0 12 24 36 48 60 72 84 96 Incubation time (hours) Biomass (g/L) The major part of this effluent is known as skim latex which comprised of 30% rubber particles while the remaining liquids are known as skim latex serum. The serum is a clear, slightly yellowish liquid with a distinct acidic odour caused by the acid used in coagulation process (mainly sulphuric acid or formic acid). According to Omorusi (2013), an average of 45 000 litres of effluent is discharged from every 20 – 30 million tonnes of rubber per rubber factory daily containing high BOD and COD, high concentration of organic matter, suspended solids and nitrogen, high gaseous and liquid effluent which could severely damage the environment if not properly discharged. (SUBMERGED FERMENTATION OF A. ORYZAE IN SLS: 24 HOURS 36 HOURS 48 HOURS • It is the first ever attempt to use non-pathogenic fungal strains for utilization of the liquid waste from the rubber industry for the production of bio-products (enzyme). The highest biomass concentrationwas obtained after 36 h while the highest protease concentration recorded was at 12 h. BOD Purification of Protease by Column Chromatography Method M = Marker IEC = Ion-Exchange Chromatogra phy +ve = Commercial protease from A. oryzae Table of Purification Sample Volume Crude Freeze Dried Dialyzed Ion-Exchange 0 HOUR ( Protease (umoles) Biomass, BOD, COD (g/l) Industrial processing of latex concentrate discharges substantial volumes of liquid waste with high BOD and COD levels annually. The release of this effluent into the environment without proper treatment will negatively affect the ecological system which human health ultimately depends on. Therefore, the present study focuses on utilizing the rubber processing effluent for bioremediation as well as value-added production of enzymes such as protease in this particular case. 200 40 52 58. 5 Total enzyme (U) 13. 148 7. 811 6. 523 4. 333 Total protein (mg) 749. 219 940. 351 1199. 234 14. 387 Specific activity (U/mg) 0. 018 0. 005 0. 301 Fold of purification Yield (%) 1. 000 0. 473 0. 310 17. 162 100 59 50 33 • Creating a zero-waste scenario and value-added product in industrial natural rubber processing. ACKNOWLEDGEME NT The financial support provided by the Ministry of Education Malaysia (KPM) through Fundamental Research Grant Scheme (FRGS), Exploratory Research Grant Scheme (ERGS) and Research Acculturation Collaborative E (RACE) are acknowledged.
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