Introduction to the Light Microscope The microscope has
- Slides: 28
Introduction to the Light Microscope
The microscope has become one of the most recognizable symbols of science. Starting with use of a simple lens in ancient times, to the first compound microscope around 1590, and up to the microscopes you are using in 7 th grade life science, the microscope has allowed scientists to make discoveries about the “invisible world. ”
Typical Classroom Microscope
What is the purpose of a microscope? • To magnify or enlarge the image of tiny objects. • The microscopes we will use this year in science can magnify objects 40 times, 100 times, and 400 times the size of the object.
What type of microscope will we be using in class this year? • In 7 th grade science, we use a compound light microscope for most of our lab activities. • A light microscope uses light as a source of energy for viewing objects. • A compound microscope contains two lenses for magnifying a specimen at the same time, the eyepiece (ocular) and one of the objective lenses.
Eyepiece
Eyepiece • Also known as the ocular • Contains the first lens you look through usually a magnification of 10 x • Located on the top of the body tube
Objective Lenses
Objective Lenses • Used in combination with the eyepiece to provide a range of magnification • Magnification ranges from 40 x to 400 x • Located on the nosepiece at the bottom of the body tube
Nosepiece
Nosepiece • Holds the objective lenses • Rotates to enable magnification • Located at the bottom of the body tube
Arm
Arm • Supports the upper parts of the microscope • Used to carry the microscope • When carrying a microscope, always have one hand on the arm and one hand on the base. Use two hands!!
Base
Base • Supports the whole microscope • Used to carry the microscope • When carrying a microscope, always have one hand on the arm and one hand on the base. Use two hands!!
Stage
Stage • Supports the slide • The slide contains the specimen or object that you are viewing with the microscope.
Stage Clip
Stage Clip • Helps to hold the slide in place • Usually one on each side of the hole (stage opening) = 2 stage clips • The stage opening allows light to pass from the light source to the lenses.
Light Source
Light Source • Provides light necessary for viewing the specimen • Usually either a mirror or illuminator • Sends light through the stage opening to the diaphragm
Diaphragm
Diaphragm • Wheel or lever located below the stage opening • Regulates the amount of light that can enter the lenses • May need to be adjusted based on the thickness of the specimen being studied
Coarse Adjustment Knob
Coarse Adjustment Knob • Raises and lowers the stage or objective lenses • Used only when focusing the low power (4 x) objective lens
Fine Adjustment Knob
Fine Adjustment Knob • Raises and lowers the stage or objective lenses a small distance for exact focusing • Used when focusing the medium power (10 x) and high power (40 x) objective lenses
Let’s Review. . . http: //www. biologycorner. com/microquiz/
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