Introduction to The Genomics Sciences Lab Services and

Introduction to The Genomics Sciences Lab Services and Support

GSL General Mission The GSL operates as a solution center and provides CHHE and the NCSU community, collaborators, and the scientific community with access to HTS, genotyping and support services In addition to serving as a research resource, the GSL is committed to the education and training of CHHE students, faculty, and staff.

GSL MISSION for CHHE High-throughput sequencing technology at NCSU GSL will support CHHE researchers to conduct • Complete transcriptome analysis Transcriptome development Gene expression analysis) • Ch. IPseq • Gene mutation analysis • SNP analysis • DNA methylation • mi. RNA analysis • e. QTL analysis • Genotyping by Sequencing • Genome wide association studies

GSL AIMS for CHHE AIM 1. Provide expertise in study design and sample preparation. The Genomics Section will provide guidance in all areas of high-throughput sequencing services, including assistance with sample and project preparation, and implementation of protocols for sequencing on various platforms. AIM 2. Provide high-throughput sequencing services. The Genomics Section will provide high-throughput sequencing technology and instrumentation, library preparation, and consultation for all levels of experimental design, development, and assessment. AIM 3. Provide guidance and expertise on Big Data analysis. The GSL is linked to NC State’s Bioinformatics Research Center (BRC) and large dataset management and analysis will be conducted at the BRC by coordinating with the STC Bioinformatician when needed.

Support from start to finish – AIMS 1 -3 Experiment design Experiment execution Tissue collection Nucleic acid extraction Nucleic acid QC Library synthesis Sequencing Data QC Data analysis Publication

Support from start to finish – AIMS 1 -3 Experiment design Choosing the best platform(s), pooling and lane use Experiment execution Tissue collection Nucleic acid extraction Nucleic acid QC Library synthesis Sequencing Data QC Data analysis Publication Watch and learn, FREE for CHHE - Tru. Seq, Ribo. Zero, Neb. Next etc. Low input, Ultra low input

AIM 2 http: //research. ncsu. edu/gsl

AIM 2

+ Next. Seq 500!! Coming soon!

Choosing the best platform – AIM 1 Hi. Seq These are cartoons of reads!! Mi. Seq Pac. Bio

Choosing the best platform – AIM 1 Hi. Seq Mi. Seq Pac. Bio

Choosing the best platform – AIM 1 Hi. Seq Error type Low complexity/diversity Mi. Seq Pac. Bio Accuracy Starting quantity

Sanger Sequencing Is your tube Single and alone? Bring it to us! For the LOW price of $4. 00 your tube can be SEQUENCED and finally find a home! *full plates too! *free pick-up service *data delivered 4 -5 pm

Other equipment and services

http: //research. ncsu. edu/gsl