Introduction CFTR A chloride channel The cystic fibrosis

Introduction • CFTR , A chloride channel • The cystic fibrosis transmembrane conductance regulator (CFTR) is a 1480 amino acid membrane bound • glycoprotein • ABC-ATPase proteins. • two, six span membrane bound regions(TMS 1, TMS 2), ATP binding domain. • The R-domain is a unique feature of CFTR within the ABC superfamily. Biochemistry, 38 (23), 7453 -7461, 1999. 1021/bi 9903603 S 0006 -2960(99)00360 -8

P 26/P 25 • 1 CKY • Compound Mol_Id: 1; Molecule: Cystic Fibrosis Transmembrane Conductance Regulator (Cftr); Chain: A; Fragment: P 26, F 508 Mutation Region; Synonym: P 26 In H 2 O; Engineered: Yes • 1 CKZ • Compound Mol_Id: 1; Molecule: Cystic Fibrosis Transmembrane Conductance Regulator (Cftr); Chain: A; Fragment: P 25, F 508 Mutation Region; Synonym: P 25 In H 2 O; Engineered: Yes

NBD 1 • 1 XMJ • Compound: Mol_Id: 1; Molecule: Cystic Fibrosis Transmembrane Conductance Regulator; Chain: A; Fragment: Nucleotide Binding Domain One; Synonym: Cftr; Camp- Dependent Chloride Channel; Ec: 3. 6. 3. 49; Engineered: Yes; Mutation: Yes

利用NBD 1作protein and protein BLAST

任選出四條不同E-value序列 • >gi|15889958|ref|NP_355639. 1| hypothetical protein AGR_C_4902 [Agrobacterium tumefaciens str. C 58] • >gi|15988440|pdb|1 JSQ|A Chain A, Structure Of Msba From Escherichia Coli: A Homolog Of The Multidrug Resistance Atp Binding Cas • >gi|15890885|ref|NP_356557. 1| hypothetical protein AGR_L_1532 [Agrobacterium tumefaciens str. C 58] • >gi|16264640|ref|NP_437432. 1| putative ABC transporter protein, fused ATP-binding and membrane-spanning components, secretes Exp. E 1 [Sinorhizobium meliloti 1021]

Scores Table (7個序列)

Alignment

THE END