Introduction ASTGOT 1 Aspartate ketoglutarate glutamate oxaloacetate 2 Slides: 9 Download presentation Introduction AST(=GOT) 1. Aspartate + α-ketoglutarate → glutamate + oxaloacetate 2. oxaloacetate + dinitrophenylhydrazine → hydrazone 3. 생성된 colored hydrazone을 spectrophotometer로 측정 ALT(=GPT) 1. Alanine + α-ketoglutarate → glutamic acid + pyruvate 2. pyruvate + dinitrophenylhydrazine → hydrazone 3. 생성된 colored hydrazone을 spectrophotometer로 측정 GOT • GPT TransaminaseIsocitrate to alpha ketoglutarateAspargineRegulation of urea cycleTitration curves of amino acidsAstgotThree stages of glycolysisLysine titration curveTransdeamination of amino acidsMetabolisme