Introduction 2 Hydrogen peroxide pathway oxidative antioxidants damage

Introduction 2

Hydrogen peroxide pathway oxidative antioxidants damage Apoptosis 8

Method 10

Evaluation of Antioxidant Activity and Preventing DNA Damage Effect of Pomegranate Extracts by Chemiluminescence Method (通過化學發光法評估石榴提取物的抗氧化活性和防止DNA損傷作用) J. Agric. Food Chem. 2007, 55, 3134 -3140 12

Sample Pyrogallol Carbonate buffer Luminol Concentration Volume(m. L) 4μg/m. L ~ 1000 μg/m. L 10 μL 6. 25 × 10 -4 M 50 μL (p. H 10. 2) 940 μL 0. 05 M mix with Na. CO 3 buffer 原文文獻內容 13

Evaluation of the Antioxidant Activity of Different Flavonoids by the Chemiluminescence Method (化學發光法評估不同的黃酮類化合物的抗氧化活性) AAPS Pharm. Sci 2003; 5 (2) Article 20 (http: //www. pharmsci. org). 14

Sample Concentration Volume(m. L) 4μg/m. L ~ 1000 μg/m. L 10 μL Hydrogen peroxide final concentration of 5 × 10– 5 M Horseradish peroxidase final concentration of 0. 2 IU/m. L Phosphate buffer Luminol 0. 1 M(p. H 7. 4) 955 μL 2 mg/m. L mix with HPO 4 buffer 原文文獻內容 15

Results 16

DPPH assay 清除DPPH自由基能力 17

Horseradish Peroxidase–Luminol– Hydrogen Peroxide System 清除H 2 O 2自由基能力 18

Pyrogallol–Luminol System 清除超氧陰離子能力 19

Discussion 20

Thank you for your attention 23