Introducing the EASYn LC Splitless nanoflow HPLC system

Overview The EASY-n. LC™ is: • • Complete Configured Compact EASY to – install

Complete • 1 cooled autosampler – 96 or 384 well plates – Single or

EASY 2 -n. LC™ (with flow control) 2 -column AFC™ Solvent A Advanced Flow

EASY 2 -n. LC™ (with flow control) 1 -column AFC™ Solvent A Advanced Flow

EASY 2 -n. LC™ (with flow control) 2 -column 1 -column Waste 6 Copyright

Compact • Small footprint – 35 cm wide (14”) – 38 cm deep (15”)

Easy to install • Only a few cables required – contact closure – [network

Easy to learn • Logical software structure • Practice oriented work flow • Results

Easy to operate (1) • What You See Is What You Get – Simple

Easy to operate (2) • Automated maintenance scripts • Intelligent trouble shooting software •

Easy to operate (3) • Split-less, nano-flow means: • Less solvent use • Less

Example (1) • Mass spectrometer – Finnigan LCQ Classic • 6 out of 36

Example (2) • Carry-over – < 0. 003% • Sample pickup – From 0.

Summary The EASY-n. LC™ is: • A fully integrated and complete instrument • With

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Complete • 1 cooled autosampler – 96 or 384 well plates – Single or double height – Wash & Waste bottles • • 3 switching valves nano-flow sensor nano-litre pumps Built-in PC & software Copyright © Proxeon Biosystems A/S

Compact • Small footprint – 35 cm wide (14”) – 38 cm deep (15”) – 45 cm high (18”) • Touch-screen controlled – built-in computer – no keyboard or mouse required • Light – 35 kg, all inclusive 7 Copyright © Proxeon Biosystems A/S

Easy to install • Only a few cables required – contact closure – [network is optional] – Power • Software is – integrated, – pre-installed and – up-to-date • Solvent purging, air removal and column flushing is fully automated • Less than 4 hours from power up to first analytical data 8 Copyright © Proxeon Biosystems A/S

Easy to learn • Logical software structure • Practice oriented work flow • Results oriented parameters – E. g. volume & flow rates, not hardware control • Intuitive graphical user interface 9 Copyright © Proxeon Biosystems A/S

Easy to operate (1) • What You See Is What You Get – Simple design – Logical layout – Clear separation in compartments – Modular components • Easy access to all parts of instrument 10 Copyright © Proxeon Biosystems A/S

Easy to operate (3) • Split-less, nano-flow means: • Less solvent use • Less waste • Robust design & Intelligent software control means: • Less support • Less maintenance • More uptime 12 M T 1 7 8 14 15 21 22 28 29 Copyright © Proxeon Biosystems A/S W 2 9 16 23 30 T 3 10 17 24 F 4 11 18 25 S 5 12 19 26 S 6 13 20 27 M T 1 7 8 14 15 21 22 28 29 W 2 9 16 23 30 T 3 10 17 24 F 4 11 18 25 S 5 12 19 26 S 6 13 20 27

Example (1) • Mass spectrometer – Finnigan LCQ Classic • 6 out of 36 consecutive runs Pre-column – 2 cm, 75µm ID, Reprosil-Pur C 18 AQ. 5µm • Analytical column – 10 cm, 75µm ID, Reprosil-Pur C 18 -AQ. 3µm • Solvents – A: 0. 005% HFBA, 0. 4% acetic acid, water – B: 0. 005% HFBA, 0. 4% acetic acid, 90% acetonitril, water • Amount injected – 100 fmol tryptic digested BSA • HPLC gradient – 300 nl/min, 10 -100 %B in 25 minutes 13 Copyright © Proxeon Biosystems A/S

Example (2) • Carry-over – < 0. 003% • Sample pickup – From 0. 2µl to full loop in 0. 01µl steps – Loop size adjustable – RSD < 0. 5% • Peak widths (FWHM) – < 10 -15 s at 50% • Reproducibility – RT RSD < 0. 7% 14 Copyright © Proxeon Biosystems A/S

Summary The EASY-n. LC™ is: • A fully integrated and complete instrument • With true nano-flow and on-line monitoring • Designed from the ground up to be easy and intuitive to operate • A nano-LC system for the 21 st century 15 Copyright © Proxeon Biosystems A/S