Integrated Bio Diagnostics Product information Slide Angiogenesis ibidi

Integrated Bio. Diagnostics Product information µ-Slide Angiogenesis © ibidi Gmb. H, Version 1. 0, January 24 th, Elias Horn 1

What is angiogenesis? Definition: Formation of new blood vessels to tumors Aim: Hinder the new vessels and you hinder tumor growth! Assays: Tube formation assay © ibidi Gmb. H Sprouting assay 2

In vitro angiogenesis assays I Tube formation assay: Cells cultivated on a gel matrix form tubes and nodes. © ibidi Gmb. H 3

In vitro angiogenesis assays II Sprouting assay: Cell spheroids or aortic rings cultivated on a gel matrix form sprouts. © ibidi Gmb. H 4

Features I µ-Slide Angiogenesis § 15 wells with numbers and letters § 4 mm wells in 5 mm wells § Inner well for 10 µl gel matrix § Upper well for 50 µl medium § Standard slide format § Lid for low evaporation § Compatible with multi-channel pipettes © ibidi Gmb. H 5

Features II § Excellent optical properties for microscopy § Homogeneous cell growth § Compatible with staining and fixation § Biocompatible plastic material – no glue, no leaking § For various types of gels (Matrigel. TM, collagen, agarose)* *Please note: The gel matrix is not part of the product! © ibidi Gmb. H 6

Filling and handling Short protocol for µ-Slide Angiogenesis Gel pipetting Gel polymerization © ibidi Gmb. H Cell seeding Experiment 7

Optical improvement I No meniscus formation in µ-Slide Angiogenesis Meniscus in 96 well plate Depth of focus All cells in focus © ibidi Gmb. H Only few cells in focus 8

Optical improvement II No meniscus formation in µ-Slide Angiogenesis Meniscus in 96 well plate Source: Peter Nelson, LMU, Munich, Germany All cells in focus © ibidi Gmb. H Only few cells in focus 9

Angiogenesis assays in µ-Slide Angiogenesis vs. 96 well plate µ-Slide Angiogenesis 96 well plate § 10 µl gel per well* § 100 µl gel per well* § No meniscus § Meniscus formation § Excellent optics § Poor optics § 15 wells § 96 wells § Slide format § Multi well format *Saves 90% gel per assay. In case of BD Matrigel. TM the costs are reduced from approx. 3€ to 0. 3€ per well/assay. © ibidi Gmb. H 10

Microscopy properties I Cells on gel matrix Cells in gel matrix Only for objective lenses with working distances over 1 mm © ibidi Gmb. H Cells without gel matrix For all objective lenses (up to 100 x) and all microscopy techniques 11

Microscopy properties II 10 µl gel create a plane surface ~ 5 µl gel create a bent surface § Good optics § Mini-well for few cells § Homogeneous cell distribution § Cell accumulation in the center © ibidi Gmb. H 12

Applications and assays § Cells on or in gels (e. g. BD Matrigel. TM) § Tube formation § Sprouting assays § Aortic ring assay § Cell spheroids § General cell culture © ibidi Gmb. H Source: Peter Nelson, LMU, Munich, Germany 13