Instrument tune page Instrument options and Toolbar icons

* Instrument tune page Instrument options and Toolbar icons Lens Tuning Controls Peak Display Other Instrument Tune Controls Waters

* n * Tuning tips Tuning instrument menus Source tune (set desired source temp, electron energy and trap then adjust lens sliders from top to bottom and repeat) n Analyser tune (Lenses 1/Lenses 2 - adjust lens sliders from top to bottom and repeat) n Lens order for tuning (from source to detector). n Lens relationships n Beam Centre/Y Deflect-1 n Rotates/Z Deflects n Waters

* This tune page displays the acceptable optimum operational * lens positions. Ion Repeller slightly 1 to the left, 90% Intensity 10%negative, Signal. Focus shape. Beam Centre near the middle and Focus 2 to the right. Source tune Electron Energy setting may require refining depending on analysis. Low (33 -35 e. V) = M+ (GC). Intermediate (35 -50 e. V) = GC/CI High (70 e. V) = Magnet scan (library search). The optimum point of these Focus 1 slider value is different to other lenses will be greatly affected lenses, since. Beam it represents the interplay voltage Centre has when introducing the GC difference betweenwith the Source (A/V) Deflect 1 theand column and. Ychanging Focus 1 lens. Electronion Energy value. Focus 2 optimizes beam at Source slit Waters Source temperature should normally be within <20 to 30 OC of end-run GCTrap column temperature. Current can be raised if high sensitivity is required.

* This tune page displays the acceptable optimum operational * 70% Intensity 30% Signal shape. If signal not significantly lens positions. Deflect lenses should optimize at or near the increased when adjusting Deflects (>10 to 15%) leave at 0. middle (exception is Y Deflect-1) and Z-Focus’s at any value. Analyser tune – Coarse tune NB - Y Deflect has relationship with Beam Centre Z Deflect 1 has relationship with Z Deflect 2 and 3 (plus Rotates) Z Focus 3 is normally set to 0 V when tuning to high resolution Waters

* 10% Intensity 90% Signal shape. If signal not significantly* Analyser tune Fine tune increased when adjusting lenses–(>10 to 15%) leave at 0. No need to adjust at low resolution. Y Focus (‘master resolution’) lens has relationship with both Curves Waters Rotate 2 has relationship with Rotate 3 and 4 (plus Z Deflects) Curve 2 has relationship with Curve 3 (and Y Focus)

* * Selected tank temperature should a steady Capillary line temperatures should allow be within at set values 30 OC Inlets Tune reference ion signal over 12 column hours (<¾ drop in for 20 minutes of upper beforeoven the GC temperature. is introduced. intensity). Waters Septum temperature can be set between 140 to 180 OC depending on desired reference signal intensity

* * This Readback slider adjusts is 100 current, = fully both in open the Amps, Accelerating flowing Control 1 units. Readback is in k. V and ESA around 10 voltages = fully magnet closed automatically. coils. Accelerating or Source Voltage slider Source and Collector Slit sliders Waters Magnet Mass slider

* Detector Z 4 Energy Restrict voltage 100 and = link (-’ve) fully scanning is open applied generally to first Ion adjusts Accelerating voltage only Control 2 plate not used 10 within fully normal thetoclosed PMT tuning. tube. (should never be=in used tune instrument). Waters

* * This Isolation Switching can be valve used Source control to reset ion will gauge board depend on (intensity allows if trip Vacuum power drops to Upper @100 X levels. Source are when met. Control crashed). PCB. Source and Analyser Ion Gauge Controls Source and Analyser Isolation Valve Controls

* Instrument Options and Toolbar PCI Board constant should setafter by Waters Magnet Field Trip Control status requires will option switching reveal onlyifbe available magnet on has when every Engineer (only affects icons undergone EPC Hall thermal Probe power or. Magnet fitted. overcurrent reset. scanning). trip. * Magnet Controls PCI Board Constant Waters

* These values should be set by Waters Instrument Options and Toolbar Engineer and no adjustment is recommended. icons Waters