Information in Biology SYNTHETIC BIOLOGY Mati Cohen Outline
Information in Biology SYNTHETIC BIOLOGY Mati Cohen
Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”
Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”
Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”
What is synthetic biology? The design and construction of new biological parts, devices, and systems The “Repressilator” The re-design of existing, natural biological systems for useful purposes. The “Diverter”
Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”
Biological - Clocks Three questions 1. How does it work 2. How does it synchronize 3. How does it translate to activity
Biological - Clocks
Biological - Clocks
Biological - Clocks
Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”
The “Repressilator” Movie
Repressilator oscillations are favored by Strong promoters Efficient ribosome-binding sites Tight transcriptional repression Comparable protein and m. RNA decay rates
Repressilator
Repressilator
Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”
Signal Transduction Movie
The Yeast system Scaffold proteins Mediating recruitment Improve efficiency of signal transfer. Facilitate interactions among different signal pathways Control localization of signal proteins within a cell.
Outline What is synthetic biology? Biological Clocks The “Repressilator” Signal transduction The “Diverter”
The Pre-Diverter
The Diverter
Diverter results
Diverter results
Summary What is synthetic biology? The design and construction of new biological parts, devices, and systems The Repressilator The re-design of existing, natural biological systems for useful purposes. The Diverter
Signal transduction movies signal transduction http: //www. youtube. com/watch? v=t. MMr TRn. Fd. I 4 signal transduction http: //www. rpi. edu/dept/bcbp/molbioche m/MBWeb/mb 1/part 2/signals. htm#anim at 2
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