Influence of magnetic fields on interfacial phenomena Malinowska

Influence of magnetic fields on interfacial phenomena Malinowska I. , Studziński M. , Malinowski H*. Faculty of Chemistry, Department of Planar Chromatography, M. Curie. Skłodowska University, M. Curie-Skłodowska Sq. 3, 20 -031 Lublin , Poland, irena. malinowska@poczta. umcs. lublin. pl Joint Institute for Nuclear Research, Veksler and Baldin Laboratory of High Energy Physics, Dubna, Russia NICA days 2015 1

Why this topic? MF Nica Days 2015 2

magnetic field in medicine Nica Days 2015 3

magnetic field and magnetic nanoparticles Nica Days 2015 4

magnetic filed and magnetic nanoparticles Nica Days 2015 5

Magnetic nanoparticle could stop blood clot caused strokes Nanotechnology News ID: 112441 Publish Date: Tue, 24 Feb 2015 15: 37: 49 GMTService: Healt Nica Days 2015 6

How magnetic field influence interfacial phenomena CHROMATOGRAPHY Nica Days 2015 7

Why chromatography? Chromatography: Method based on different interactions of the solutes with stationary phase surface and components of the mobile phase The different interactions are caused by different properties of the solutes Nica Days 2015 8

physical – GPC/SEC chemical structure presence of functional groups acid/base properties interactions • hydrophilic (HILIC) • hydrophobic (HIC) • electrostatic – ICC, • witch metal cations - chiral chromatography, argentation chromatography • with stationary phase - NP, RP chromatography Nica Days 2015 9

Chromatography in magnetic field can give us some information about influencing of magnetic fields interphacial phenomena. Nica Days 2015 10

chromatography in magnetic fieldmagnetochromatography analytical Fundamental research Does magnetic field influence the solute retention and efficiency of chromatographic systems? Does magnetic field influence interphacial phenomena? Does magnetic field influence properies of solutes? Separation results? Nica Days 2015 11

Chromatography in magnetic field sources of magnetic field superconducting magnet neodymium magnet B: 0. 5 -3. 5 T B= 0. 48 T Nica Days 2015 12

Chelidonium maius L. Tertiary alkaliods Quaternary alkaloids Nica Days 2015 13

extract of tertiary alkaloids MF Stationary phase Si. O 2 Mobile phae: toluene/ethyl acetate /methanol 70/15/15 v/v/v Nica Days 2015 14

MF Nica Days 2015 15

Quaternary alkaloid extract. Nica Days 2015 16

amino acids in magnetic field RF=a/b Nica Days 2015 17

Investigated compounds – heteronuclear complexes f-electron elements Nica Days 2015 18

Heteronuclear complexes in magnetic filed Nica Days 2015 19

PAH resorcynothiadiazole derivatives Nica Days 2015 20

PAH B= 1. 87 T, sationary phase Si. O 2 60, mobile phase – n-hexane Nica Days 2015 21

PAH retention in strong magnetic fields RF=a/b Nica Days 2015 22

Rezorcynotiadiazole -NP MF 0, 0. 56, 1. 87, 3. 1 T Nica Days 2015 23

aminoacids B=1. 84 T Nica Days 2015 24

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important questions 1 2 3 • does magnetic field influence interaction of drugs with receptors • does magnetic field influence penetrability of the drugs through biological membranes? • does magnetic field influence some processes in nature? Nica Days 2015 26

CHROMATOGRAPHY Nica Days 2015 27

lipophilicity and magnetic field Partition coefficient n-octanolwater Nica Days 2015 28

correlation between log P and log K Correlation betweeen relation preameability velocity of substance (log. K) through biological membrane alga Chara ceratophylla and partition coefficient oil/water (log. P) Nica Days 2015 29

Chromatographic system imitatating biological membrane Stationary phase : C-18 mobile phase: water RM = RM 0 -s φ Nica Days 2015 30

3 2. 5 2 1. 5 logkw(MF) logkw 1 0. 5 0 1 R 2 R 3 R 4 R B=1. 98 T Nica Days 2015 31

complexes Nica Days 2015 32

biogenic amines in magnetic field Nica Days 2015 33

MLC Micellar Liquid Chromatography Stationary phase– C-18 Mobile phase– buffer (p. H=7. 4)-organic modifier, surfactant – concentration above CMC Nica Days 2015 34

BMC - Biopartitioning Micellar Chromatography Stationary phase– C-18 organic modifier– iso-buthanol surfactant Nica Days 2015 35

MLC of biogenic amines in magnetic field Nica Days 2015 36

MLC - determiantion of physicochemical parameters 1/k Foley’s equation adrenalina 0. 24 R 2 = 0. 9966 0. 16 0. 08 0 0 Kma – asociation constant analite – micelle, 0. 05 CM [M] 0. 1 Psw – partition coefficient of analyte between stationary phase and water, Φ – volume ratio of stationary phase (Vs) /mobile phase(Vm). Nica Days 2015 37

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why the investigations? change of lipophilicity of substance change migration velocity of substane change concentration profile of substance VA >VB > VC Nica Days 2015 39

L • liberation A • absorption D • distribution M • metabolism? E • excertion? Nica Days 201 40

02 -0 -1065 -2007/2014 „Development of INNR Basic Facility for Generation of Intense Heavy Ion and Polarized Nuclear Beams Aimed and Searching for the Mixed Phase of Nuclear Matter and Investigation of Polarization Phenomena at the Collision Energies to up to 11 Ge. V” 1. Malinowska I. , Studziński M. , Malinowski H. „The Effect of a Magnietic Field on the Retention of Polyaromatic Hydrocarbons in Planar Chromatography”, „JPC - Journal of Planar Chromatography Modern TLC” 2008 (21) 5 p. 379 -385 2. Malinowska I. , Studziński M. , Malinowski H. ”Some aspect of TLC in homogenous magnetic Fields” “Journal of Separation Science” 2011 vol 34 iss 15 p. 1788 -1795 3. Malinowska I. , Studziński M. , Malinowski H. „Magneto-Thin-Layer Chromatografy as a Tool for the Analysis of Plant Extracts”, „JPCJournal of Planar Chromatography Modern TLC” 27(2014)5 p. 340 -345. Nica Days 2015 41

acknowledgement scientific and organisation committee NICA days 2015 dr Mark Studziński dr H. Malinowski Nica Days 2015 42

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Futher investigations 1 2 • influence of magnetic field interaction of biological active substances with blood proteins • Magnetic stationary phase 3 • Influence of magnetic field on micelle formation 4 • Influence of magnetic filed on interaction of drugs with receptors. 5 • Influence of magnetic field kinetics of metabolism processes. Nica Days 2015 44

conclusions Magnetic field can influence on retention of chromatographic solutes The influence depends on B of magnetic field, direction of magnetic field lines in relation of mobile phase migration direction. In micellar systems magnetic field influence on hydrophobicity and interactions of chromatographic solutes with micelles. Nica Days 2015 45

retention differences in perpendicular and paralell magnetic field WWA resorcynthiadiazoles Nica Days 2015 46