Induction of adult levels of globin in human

Induction of adult levels of β-globin in human erythroid cells that intrinsically express embryonic or fetal globin by transduction with KLF 1 and BCL 11 A-XL by Kongtana Trakarnsanga, Marieangela C. Wilson, Winnie Lau, Belinda K. Singleton, Steve F. Parsons, Punthita Sakuntanaga, Ryo Kurita, Yukio Nakamura, David J. Anstee, and Jan Frayne haematol Volume 99(11): 1677 -1685 November 3, 2014 © 2014 by Ferrata Storti Foundation

Co-transfection of K 562 cells with KLF 1 and BCL 11 A-XL induces β-globin expression. Kongtana Trakarnsanga et al. Haematologica 2014; 99: 16771685 © 2014 by Ferrata Storti Foundation

Expression of KLF 1, BCL 11 A and globin subunits by erythroid cells differentiated in vitro from adult, cord blood and C 19 i. PSC progenitors and Hi. DEP-1 cells. Kongtana Trakarnsanga et al. Haematologica 2014; 99: 16771685 © 2014 by Ferrata Storti Foundation

Induced expression of β-globin in Hi. DEP-1 cells transduced with BCL 11 A-XL. Kongtana Trakarnsanga et al. Haematologica 2014; 99: 16771685 © 2014 by Ferrata Storti Foundation

Expression of β-globin in Hi. DEP-1 erythroid cells transduced with e. GFP-BCL 11 A-XL. Kongtana Trakarnsanga et al. Haematologica 2014; 99: 16771685 © 2014 by Ferrata Storti Foundation

Level of β-, γ- and ε-globin in Hi. DEP-1 cells before and after transduction with e. GFP-BCL 11 A-XL. Kongtana Trakarnsanga et al. Haematologica 2014; 99: 16771685 © 2014 by Ferrata Storti Foundation

Comparison of the level of globin subunits in Hi. DEP-1 cells before and after transduction with BCL 11 A-XL and adult erythroid cells. Kongtana Trakarnsanga et al. Haematologica 2014; 99: 16771685 © 2014 by Ferrata Storti Foundation

Co-transfection of erythroid cells differentiated in vitro from cord blood progenitors with KLF 1 and BCL 11 A-XL increases β-globin expression. Kongtana Trakarnsanga et al. Haematologica 2014; 99: 16771685 © 2014 by Ferrata Storti Foundation