Fusion protein The green fluorescent protein (GFP) is composed of 238 amino acids (26. 9 k. Da), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light.
Figure 25. 05
GFP derivatives In 2008, Osamu Shimomura, Marty Chalfie and Roger Tsien (錢永建) won the Nobel Prize in chemistry.
重組蛋白質 在寄主(host)細胞中的表達 基因 優點 • 產量大 • 操作易 質體 染色體 4, 000 kb E. coli 缺點 • no post-translation modification • inclusion body • not secrete protein
重組蛋白質 在寄主細胞中的表達 E. coli 缺點 not secrete protein gram-positive bacteria B. subtilis proteases limited vector & promoter “signal” has not found yet